TY - JOUR
T1 - Self-powered integrated microfluidic point-of-care low-cost enabling (SIMPLE) chip
AU - Yeh, Erh Chia
AU - Fu, Chi Cheng
AU - Hu, Lucy
AU - Thakur, Rohan
AU - Feng, Jeffrey
AU - Lee, Luke P.
N1 - Funding Information:
We thank M. Y. Huang for providing technical assistance and K. Feng for the computer animations. We thank P. Lum at the Biomolecular Nanotechnology Center for technical assistance. We also thank the BIOPOETS lab members for providing valuable feedback. The HIV primers for RPA were supplied by O. Piepenburg and N. Armes (TwistDx, U.K.). Funding: This work was funded by the Defence Advanced Research Projects Agency HR0011-12-2-0003 and the Bill & Melinda Gates Foundation through the Grand Challenges in Global Health Initiative OPP1028785.
Publisher Copyright:
© 2017 The Authors, some rights reserved.
PY - 2017/3
Y1 - 2017/3
N2 - Portable, low-cost, and quantitative nucleic acid detection is desirable for point-of-care diagnostics; however, current polymerase chain reaction testing often requires time-consuming multiple steps and costly equipment. We report an integrated microfluidic diagnostic device capable of on-site quantitative nucleic acid detection directly from the blood without separate sample preparation steps. First, we prepatterned the amplification initiator [magnesium acetate (MgOAc)] on the chip to enable digital nucleic acid amplification. Second, a simplified sample preparation step is demonstrated, where the plasma is separated autonomously into 224 microwells (100 nl per well) without any hemolysis. Furthermore, self-powered microfluidic pumping without any external pumps, controllers, or power sources is accomplished by an integrated vacuum battery on the chip. This simple chip allows rapid quantitative digital nucleic acid detection directly from human blood samples (10 to 105 copies of methicillin-resistant Staphylococcus aureus DNA per microliter, ~30 min, via isothermal recombinase polymerase amplification). These autonomous, portable, lab-on-chip technologies provide promising foundations for future low-cost molecular diagnostic assays.
AB - Portable, low-cost, and quantitative nucleic acid detection is desirable for point-of-care diagnostics; however, current polymerase chain reaction testing often requires time-consuming multiple steps and costly equipment. We report an integrated microfluidic diagnostic device capable of on-site quantitative nucleic acid detection directly from the blood without separate sample preparation steps. First, we prepatterned the amplification initiator [magnesium acetate (MgOAc)] on the chip to enable digital nucleic acid amplification. Second, a simplified sample preparation step is demonstrated, where the plasma is separated autonomously into 224 microwells (100 nl per well) without any hemolysis. Furthermore, self-powered microfluidic pumping without any external pumps, controllers, or power sources is accomplished by an integrated vacuum battery on the chip. This simple chip allows rapid quantitative digital nucleic acid detection directly from human blood samples (10 to 105 copies of methicillin-resistant Staphylococcus aureus DNA per microliter, ~30 min, via isothermal recombinase polymerase amplification). These autonomous, portable, lab-on-chip technologies provide promising foundations for future low-cost molecular diagnostic assays.
UR - http://www.scopus.com/inward/record.url?scp=85021683288&partnerID=8YFLogxK
U2 - 10.1126/sciadv.1501645
DO - 10.1126/sciadv.1501645
M3 - Article
C2 - 28345028
AN - SCOPUS:85021683288
SN - 2375-2548
VL - 3
JO - Science Advances
JF - Science Advances
IS - 3
M1 - e1501645
ER -