Screening of target-specific olfactory receptor and development of olfactory biosensor for the assessment of fungal contamination in grain

Jung Ho Ahn, Jong Hyun Lim, Juhun Park, Eun Hae Oh, Manki Son, Seunghun Hong, Tai Hyun Park

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

We herein report an integrated olfactory system to carbon nanotube platforms for biosensing applications. In particular, this system can be used for the real-time monitoring of fungal contamination in grain through detecting 1-octen-3-ol, which is specifically generated from contaminated grain. A specific human olfactory receptor (OR) that recognizes 1-octen-3-ol was found using a cyclic adenosine monophosphate (cAMP) response element (CRE)-reporter gene assay. Then, OR-containing nanovesicles were produced from human embryonic kidney (HEK)-293 cells. The nanovesicles, which generate olfactory signals using endogenous cellular components and over-expressed ORs, were integrated into single-walled carbon nanotubes field-effect transistors (SWNT-FETs). The nanovesicles and SWNT-FETs play roles in perceiving specific odorants, and in amplifying cellular signals, respectively. Thus, the nanovesicle-integrated device was able to detect 1-octen-3-ol with excellent sensitivity and selectivity, similar to the original olfactory system. This system can be effectively utilized for the real-time measurement of fungal contamination in grain.

Original languageEnglish
Pages (from-to)9-16
Number of pages8
JournalSensors and Actuators, B: Chemical
Volume210
DOIs
StatePublished - Apr 2015

Bibliographical note

Funding Information:
This study was supported by the National Research Foundation of Korea (NRF) grant funded by the Ministry of Science , ICT & Future Planning. THP appreciates the support from the NRF grants (No. 2014039771 , No. 2014053108 ). SH acknowledges the support from the NRF grants (No. 2013M3A6B2078958 , No. 2014M3A7B4051591 ). This work was supported by the Seoul National University Brain Fusion Program Research Grant (No. 800-20130440 ).

Publisher Copyright:
© 2014 Elsevier B.V. All rights reserved.

Keywords

  • 1-Octen-3-ol
  • Bioelectronic nose
  • Carbon nanotube
  • Fungal contamination
  • Nanovesicle

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