Abstract
We herein report an integrated olfactory system to carbon nanotube platforms for biosensing applications. In particular, this system can be used for the real-time monitoring of fungal contamination in grain through detecting 1-octen-3-ol, which is specifically generated from contaminated grain. A specific human olfactory receptor (OR) that recognizes 1-octen-3-ol was found using a cyclic adenosine monophosphate (cAMP) response element (CRE)-reporter gene assay. Then, OR-containing nanovesicles were produced from human embryonic kidney (HEK)-293 cells. The nanovesicles, which generate olfactory signals using endogenous cellular components and over-expressed ORs, were integrated into single-walled carbon nanotubes field-effect transistors (SWNT-FETs). The nanovesicles and SWNT-FETs play roles in perceiving specific odorants, and in amplifying cellular signals, respectively. Thus, the nanovesicle-integrated device was able to detect 1-octen-3-ol with excellent sensitivity and selectivity, similar to the original olfactory system. This system can be effectively utilized for the real-time measurement of fungal contamination in grain.
| Original language | English |
|---|---|
| Pages (from-to) | 9-16 |
| Number of pages | 8 |
| Journal | Sensors and Actuators, B: Chemical |
| Volume | 210 |
| DOIs | |
| State | Published - Apr 2015 |
Bibliographical note
Funding Information:This study was supported by the National Research Foundation of Korea (NRF) grant funded by the Ministry of Science , ICT & Future Planning. THP appreciates the support from the NRF grants (No. 2014039771 , No. 2014053108 ). SH acknowledges the support from the NRF grants (No. 2013M3A6B2078958 , No. 2014M3A7B4051591 ). This work was supported by the Seoul National University Brain Fusion Program Research Grant (No. 800-20130440 ).
Publisher Copyright:
© 2014 Elsevier B.V. All rights reserved.
Keywords
- 1-Octen-3-ol
- Bioelectronic nose
- Carbon nanotube
- Fungal contamination
- Nanovesicle