Scopoletin from the flower buds of Magnolia fargesii inhibits protein glycation, aldose reductase, and cataractogenesis Ex Vivo

Jun Lee; Nan Hee Kim; Joo Won Nam; Yun Mi Lee; Dae Sik Jang; Young Sook Kim; Sang Hae Nam; Eun Kyoung Seo; Min Suk Yang; Jin Sook Kim

doi:10.1007/s12272-010-0904-z

Scopoletin from the flower buds of Magnolia fargesii inhibits protein glycation, aldose reductase, and cataractogenesis Ex Vivo

Jun Lee, Nan Hee Kim, Joo Won Nam, Yun Mi Lee, Dae Sik Jang, Young Sook Kim, Sang Hae Nam, Eun Kyoung Seo, Min Suk Yang, Jin Sook Kim

Department of Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

52 Scopus citations

Abstract

Five compounds previously known structures, scopoletin (1), northalifoline (2), stigmast-4-en-3-one (3), tiliroside (4), and oplopanone (5) were obtained from the flower buds of Magnolia fargesii using chromatographic separation methods. The structures of 1-5 were identified by the interpretation of their spectroscopic data including 1D- and 2D-NMR as well as by comparison with reported values. Three compounds 1-3 were found from M. fargesii for the first time in this study. All the isolates (1-5) were subjected to in vitro bioassays to evaluate the inhibitory activity on advanced glycation end products formation and rat lens aldose reductase (RLAR). Compound 1 showed a remarkable inhibitory activity on advanced glycation end products formation with IC₅₀ value of 2.93 μM (aminoguanidine: 961 μM), and showed a significant RLAR inhibitory activity with IC₅₀ value of 22.5 μM (3.3-tetramethyleneglutaric acid: 28.7 μM). Compound 4 exhibited potent inhibitory activity against RLAR (IC₅₀ = 14.9 μM). In the further experiment ex vivo, cataractogenesis of rat lenses induced with xylose was significantly inhibited by compound 1 treatment.

Original language	English
Pages (from-to)	1317-1323
Number of pages	7
Journal	Archives of Pharmacal Research
Volume	33
Issue number	9
DOIs	https://doi.org/10.1007/s12272-010-0904-z
State	Published - Sep 2010

Bibliographical note

Funding Information:
This research was supported by a grant [L08010 and K09030] from Korea Institute of Oriental Medicine. NMR and MS experiments were performed by the Korea Basic Science Institute.

Keywords

Advanced glycation end products
Cataractogenesis
Diabetic complications
Magnolia fargesii
Rat lens aldose reductase
Scopoletin

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s12272-010-0904-z

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title = "Scopoletin from the flower buds of Magnolia fargesii inhibits protein glycation, aldose reductase, and cataractogenesis Ex Vivo",

abstract = "Five compounds previously known structures, scopoletin (1), northalifoline (2), stigmast-4-en-3-one (3), tiliroside (4), and oplopanone (5) were obtained from the flower buds of Magnolia fargesii using chromatographic separation methods. The structures of 1-5 were identified by the interpretation of their spectroscopic data including 1D- and 2D-NMR as well as by comparison with reported values. Three compounds 1-3 were found from M. fargesii for the first time in this study. All the isolates (1-5) were subjected to in vitro bioassays to evaluate the inhibitory activity on advanced glycation end products formation and rat lens aldose reductase (RLAR). Compound 1 showed a remarkable inhibitory activity on advanced glycation end products formation with IC50 value of 2.93 μM (aminoguanidine: 961 μM), and showed a significant RLAR inhibitory activity with IC50 value of 22.5 μM (3.3-tetramethyleneglutaric acid: 28.7 μM). Compound 4 exhibited potent inhibitory activity against RLAR (IC50 = 14.9 μM). In the further experiment ex vivo, cataractogenesis of rat lenses induced with xylose was significantly inhibited by compound 1 treatment.",

keywords = "Advanced glycation end products, Cataractogenesis, Diabetic complications, Magnolia fargesii, Rat lens aldose reductase, Scopoletin",

author = "Jun Lee and Kim, {Nan Hee} and Nam, {Joo Won} and Lee, {Yun Mi} and Jang, {Dae Sik} and Kim, {Young Sook} and Nam, {Sang Hae} and Seo, {Eun Kyoung} and Yang, {Min Suk} and Kim, {Jin Sook}",

note = "Funding Information: This research was supported by a grant [L08010 and K09030] from Korea Institute of Oriental Medicine. NMR and MS experiments were performed by the Korea Basic Science Institute.",

year = "2010",

month = sep,

doi = "10.1007/s12272-010-0904-z",

language = "English",

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T1 - Scopoletin from the flower buds of Magnolia fargesii inhibits protein glycation, aldose reductase, and cataractogenesis Ex Vivo

AU - Lee, Jun

AU - Kim, Nan Hee

AU - Nam, Joo Won

AU - Lee, Yun Mi

AU - Jang, Dae Sik

AU - Kim, Young Sook

AU - Nam, Sang Hae

AU - Seo, Eun Kyoung

AU - Yang, Min Suk

AU - Kim, Jin Sook

N1 - Funding Information: This research was supported by a grant [L08010 and K09030] from Korea Institute of Oriental Medicine. NMR and MS experiments were performed by the Korea Basic Science Institute.

PY - 2010/9

Y1 - 2010/9

N2 - Five compounds previously known structures, scopoletin (1), northalifoline (2), stigmast-4-en-3-one (3), tiliroside (4), and oplopanone (5) were obtained from the flower buds of Magnolia fargesii using chromatographic separation methods. The structures of 1-5 were identified by the interpretation of their spectroscopic data including 1D- and 2D-NMR as well as by comparison with reported values. Three compounds 1-3 were found from M. fargesii for the first time in this study. All the isolates (1-5) were subjected to in vitro bioassays to evaluate the inhibitory activity on advanced glycation end products formation and rat lens aldose reductase (RLAR). Compound 1 showed a remarkable inhibitory activity on advanced glycation end products formation with IC50 value of 2.93 μM (aminoguanidine: 961 μM), and showed a significant RLAR inhibitory activity with IC50 value of 22.5 μM (3.3-tetramethyleneglutaric acid: 28.7 μM). Compound 4 exhibited potent inhibitory activity against RLAR (IC50 = 14.9 μM). In the further experiment ex vivo, cataractogenesis of rat lenses induced with xylose was significantly inhibited by compound 1 treatment.

AB - Five compounds previously known structures, scopoletin (1), northalifoline (2), stigmast-4-en-3-one (3), tiliroside (4), and oplopanone (5) were obtained from the flower buds of Magnolia fargesii using chromatographic separation methods. The structures of 1-5 were identified by the interpretation of their spectroscopic data including 1D- and 2D-NMR as well as by comparison with reported values. Three compounds 1-3 were found from M. fargesii for the first time in this study. All the isolates (1-5) were subjected to in vitro bioassays to evaluate the inhibitory activity on advanced glycation end products formation and rat lens aldose reductase (RLAR). Compound 1 showed a remarkable inhibitory activity on advanced glycation end products formation with IC50 value of 2.93 μM (aminoguanidine: 961 μM), and showed a significant RLAR inhibitory activity with IC50 value of 22.5 μM (3.3-tetramethyleneglutaric acid: 28.7 μM). Compound 4 exhibited potent inhibitory activity against RLAR (IC50 = 14.9 μM). In the further experiment ex vivo, cataractogenesis of rat lenses induced with xylose was significantly inhibited by compound 1 treatment.

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KW - Cataractogenesis

KW - Diabetic complications

KW - Magnolia fargesii

KW - Rat lens aldose reductase

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SN - 0253-6269

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SP - 1317

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JO - Archives of Pharmacal Research

JF - Archives of Pharmacal Research

IS - 9

ER -

Scopoletin from the flower buds of Magnolia fargesii inhibits protein glycation, aldose reductase, and cataractogenesis Ex Vivo

Abstract

Bibliographical note

Keywords

UN SDGs

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