Roll-to-roll fabrication of integrated PDMS-paper microfluidics for nucleic acid amplification

Jussi Hiltunen, Christina Liedert, Marianne Hiltunen, Olli Heikki Huttunen, Johanna Hiitola-Keinänen, Sanna Aikio, Mikko Harjanne, Marika Kurkinen, Leena Hakalahti, Luke P. Lee

Research output: Contribution to journalArticlepeer-review

62 Scopus citations


Microfluidic-based integrated molecular diagnostic systems, which are automated, sensitive, specific, user-friendly, robust, rapid, easy-to-use, and portable, can revolutionize future medicine. Current research and development largely relies on polydimethylsiloxane (PDMS) to fabricate microfluidic devices. Since the transition from the proof-of-principle phase to clinical studies requires a vast number of integrated microfluidic devices, there is a need for a high-volume manufacturing method of silicone-based microfluidics. Here we present the first roll-to-roll (R2R) thermal imprinting method to fabricate integrated PDMS-paper microfluidics for molecular diagnostics, which allows production of tens of thousands of replicates in an hour. In order to validate the replicated molecular diagnostic platforms, on-chip amplification of viral ribonucleic acid (RNA) with loop-mediated isothermal amplification (LAMP) was demonstrated. These low-cost, rapid and accurate molecular diagnostic platforms will generate a wide range of applications in preventive personalized medicine, global healthcare, agriculture, food, environment, water monitoring, and global biosecurity.

Original languageEnglish
Pages (from-to)1552-1559
Number of pages8
JournalLab on a Chip
Issue number11
StatePublished - 7 Jun 2018


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