Roll-to-roll fabrication of integrated PDMS-paper microfluidics for nucleic acid amplification

Jussi Hiltunen, Christina Liedert, Marianne Hiltunen, Olli Heikki Huttunen, Johanna Hiitola-Keinänen, Sanna Aikio, Mikko Harjanne, Marika Kurkinen, Leena Hakalahti, Luke P. Lee

Research output: Contribution to journalArticlepeer-review

68 Scopus citations

Abstract

Microfluidic-based integrated molecular diagnostic systems, which are automated, sensitive, specific, user-friendly, robust, rapid, easy-to-use, and portable, can revolutionize future medicine. Current research and development largely relies on polydimethylsiloxane (PDMS) to fabricate microfluidic devices. Since the transition from the proof-of-principle phase to clinical studies requires a vast number of integrated microfluidic devices, there is a need for a high-volume manufacturing method of silicone-based microfluidics. Here we present the first roll-to-roll (R2R) thermal imprinting method to fabricate integrated PDMS-paper microfluidics for molecular diagnostics, which allows production of tens of thousands of replicates in an hour. In order to validate the replicated molecular diagnostic platforms, on-chip amplification of viral ribonucleic acid (RNA) with loop-mediated isothermal amplification (LAMP) was demonstrated. These low-cost, rapid and accurate molecular diagnostic platforms will generate a wide range of applications in preventive personalized medicine, global healthcare, agriculture, food, environment, water monitoring, and global biosecurity.

Original languageEnglish
Pages (from-to)1552-1559
Number of pages8
JournalLab on a Chip
Volume18
Issue number11
DOIs
StatePublished - 7 Jun 2018

Bibliographical note

Funding Information:
The authors acknowledge financial support by a Fulbright Scholarship for Luke P. Lee's proposal on “Intelligent paper opto-electro-microfluidic systems (iPOEMs) for preventive personalized medicine and global healthcare,” and the Academy of Finland grant No. 284907. Jaakko Pennanen, Ulla Sarajärvi, Pekka Ontero and Jenni Tomperi at VTT are acknowledged for their assistance in R2R fabrication. Janne Aikio and Antti Veijola at VTT-Technical Research Centre of Finland are acknowledged for filming and taking the photographs of the PDMS samples. We thank Topi Hintsala, Ginolis Ltd, for his assistance with primer dispensing.

Publisher Copyright:
© 2018 The Royal Society of Chemistry.

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