Rifampicin interference in the measurement of urinary catecholamines by high-performance liquid chromatography

Hyun Ki Kim, Dae Hyun Ko, Tae Dong Jeong, Sang Hee Lee, Woochang Lee, Soo Youn Lee, Sail Chun, Junghan Song, Won Ki Min

Research output: Contribution to journalArticlepeer-review

Abstract

Measurement of urine and plasma catecholamines and their metabolites are essential for the diagnosis of pheochromocytoma. Here, we report our experience of rifampicin interference in the measurement of urinary catecholamines using high-performance liquid chromatography. During a 1-year period, four patients taking rifampicin showed unusual chromatograms with markedly larger norepinephrine spikes, translating to highly elevated norepinephrine concentrations: 290.2, 720.1, 312.0, and 812.7 μg/L. This interference effect did not occur when samples were prepared using the alumina method, with norepinephrine values decreasing to 26.4, 31.5, 21.9, and 17.2 μg/L. Interference was also absent in data using LC-MS/MS. Clinical laboratories should verify whether rifampicin interferes with test results in their assays, and physicians should be informed that urinary norepinephrine levels can be falsely elevated in patients taking rifampicin.

Original languageEnglish
Pages (from-to)356-359
Number of pages4
JournalAnnals of Clinical and Laboratory Science
Volume45
Issue number3
StatePublished - 2015

Bibliographical note

Publisher Copyright:
© 2015 by the Association of Clinical Scientists, Inc.

Keywords

  • Catecholamine
  • High-performance liquid chromatography
  • Interference
  • Rifampicin

Fingerprint

Dive into the research topics of 'Rifampicin interference in the measurement of urinary catecholamines by high-performance liquid chromatography'. Together they form a unique fingerprint.

Cite this