Abstract
Measurement of urine and plasma catecholamines and their metabolites are essential for the diagnosis of pheochromocytoma. Here, we report our experience of rifampicin interference in the measurement of urinary catecholamines using high-performance liquid chromatography. During a 1-year period, four patients taking rifampicin showed unusual chromatograms with markedly larger norepinephrine spikes, translating to highly elevated norepinephrine concentrations: 290.2, 720.1, 312.0, and 812.7 μg/L. This interference effect did not occur when samples were prepared using the alumina method, with norepinephrine values decreasing to 26.4, 31.5, 21.9, and 17.2 μg/L. Interference was also absent in data using LC-MS/MS. Clinical laboratories should verify whether rifampicin interferes with test results in their assays, and physicians should be informed that urinary norepinephrine levels can be falsely elevated in patients taking rifampicin.
| Original language | English |
|---|---|
| Pages (from-to) | 356-359 |
| Number of pages | 4 |
| Journal | Annals of Clinical and Laboratory Science |
| Volume | 45 |
| Issue number | 3 |
| State | Published - 2015 |
Bibliographical note
Publisher Copyright:© 2015 by the Association of Clinical Scientists, Inc.
Keywords
- Catecholamine
- High-performance liquid chromatography
- Interference
- Rifampicin