TY - JOUR
T1 - Rhodamine-based near-infrared probe for emission detection of ATP in lysosomes in living cells
AU - Tikum, Anjong Florence
AU - Kim, Gyoungmi
AU - Nasirian, Azam
AU - Ko, Jeong Won
AU - Yoon, Juyoung
AU - Kim, Jinheung
N1 - Funding Information:
This work was supported by the National Research Foundation (NRF) grant funded by the Korean government ( NRF-2017R1A5A1015365 ) and “Next Generation Carbon Upcycling Project” (Project No. 2017M1A2A2046740) through the NRF funded by the Ministry of Science and ICT . J.Y. acknowledges grants from the National Creative Research Initiative programs of the National Research Foundation of Korea (NRF) funded by the Korean government (MSIP) (No. 2012R1A3A2048814 ). Mass spectral data were obtained from the Korea Basic Science Institute (Daegu), by Jeol JMS 700 high-resolution mass spectrometry.
Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2019/8/1
Y1 - 2019/8/1
N2 - A new near infrared fluorescent probe was designed and prepared to detect ATP and target lysosomes in cells. The non-fluorescent probe was turned on during the interaction with ATP, affording a 115-fold increase in fluorescence intensity. The colorless probe turned purple upon interaction with ATP. The probe displayed high sensitivity and selectivity toward ATP compared with other biomolecules, such as AMP, ADP, GMP, and CMP. Such significant spectroscopic changes derive from a structural change of the probe's spirocyclic moiety to its ring-opened form. This occurs through electrostatic and π,π-stacking interactions between the probe and ATP. This probe was also used in selective lysosome staining and for real-time ATP level monitoring. It exhibits great advantages in cell imaging studies, such as excellent photostability, low cytotoxicity, and good cell membrane permeability.
AB - A new near infrared fluorescent probe was designed and prepared to detect ATP and target lysosomes in cells. The non-fluorescent probe was turned on during the interaction with ATP, affording a 115-fold increase in fluorescence intensity. The colorless probe turned purple upon interaction with ATP. The probe displayed high sensitivity and selectivity toward ATP compared with other biomolecules, such as AMP, ADP, GMP, and CMP. Such significant spectroscopic changes derive from a structural change of the probe's spirocyclic moiety to its ring-opened form. This occurs through electrostatic and π,π-stacking interactions between the probe and ATP. This probe was also used in selective lysosome staining and for real-time ATP level monitoring. It exhibits great advantages in cell imaging studies, such as excellent photostability, low cytotoxicity, and good cell membrane permeability.
KW - Adenosine triphosphate
KW - Cell imaging
KW - Fluorescence detection
KW - Fluorescent probe for ATP
KW - Lysosome targeting
UR - http://www.scopus.com/inward/record.url?scp=85064753999&partnerID=8YFLogxK
U2 - 10.1016/j.snb.2019.04.112
DO - 10.1016/j.snb.2019.04.112
M3 - Article
AN - SCOPUS:85064753999
SN - 0925-4005
VL - 292
SP - 40
EP - 47
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
ER -