A new near infrared fluorescent probe was designed and prepared to detect ATP and target lysosomes in cells. The non-fluorescent probe was turned on during the interaction with ATP, affording a 115-fold increase in fluorescence intensity. The colorless probe turned purple upon interaction with ATP. The probe displayed high sensitivity and selectivity toward ATP compared with other biomolecules, such as AMP, ADP, GMP, and CMP. Such significant spectroscopic changes derive from a structural change of the probe's spirocyclic moiety to its ring-opened form. This occurs through electrostatic and π,π-stacking interactions between the probe and ATP. This probe was also used in selective lysosome staining and for real-time ATP level monitoring. It exhibits great advantages in cell imaging studies, such as excellent photostability, low cytotoxicity, and good cell membrane permeability.
Bibliographical noteFunding Information:
This work was supported by the National Research Foundation (NRF) grant funded by the Korean government ( NRF-2017R1A5A1015365 ) and “Next Generation Carbon Upcycling Project” (Project No. 2017M1A2A2046740) through the NRF funded by the Ministry of Science and ICT . J.Y. acknowledges grants from the National Creative Research Initiative programs of the National Research Foundation of Korea (NRF) funded by the Korean government (MSIP) (No. 2012R1A3A2048814 ). Mass spectral data were obtained from the Korea Basic Science Institute (Daegu), by Jeol JMS 700 high-resolution mass spectrometry.
© 2019 Elsevier B.V.
- Adenosine triphosphate
- Cell imaging
- Fluorescence detection
- Fluorescent probe for ATP
- Lysosome targeting