Retrovirus-mediated gene transfer of the Escherichia coli LacZ gene to hepatocytes in vitro and in vivo

Sangtaek Oh, Jeongkeun Rih, Su Jeong Kim, Dongmin Kang, Deog Su Hwang, Jeongbin Yim

Research output: Contribution to journalArticlepeer-review

Abstract

A recombinant retroviral vector CRIP/MFG-LacZ containing the E. coli LacZ gene was used to examine transferring gene to liver. Various hepatocytes in vitro and mouse liver in vivo were transduced with CRIP/MFG-LacZ followed by detecting the expression of the LacZ gene through X-gal staining. Among the hepatocytes, including mouse primary hepatocyte, mouse hepatoma Hepa 1-6, immortalized human hepatocyte Chang, human hepatoma Hep G2, and human hepatoma Hep 3B, mouse primary hepatocytes were most efficiently transduced. Mouse hepatocytes were more efficiently transduced compared to human hepatocytes. The expression of LacZ was maximal at 3 to 5 days after transduction, then decreased to the basal level 7 days later. Infusion of the retrovirus through the portal vein of the mouse appeared to mostly transduce non-parenchymal cells.

Original languageEnglish
Pages (from-to)18-22
Number of pages5
JournalMolecules and Cells
Volume6
Issue number1
StatePublished - 29 Feb 1996

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