TY - JOUR
T1 - Residue Leu973 of the rat α1 subunit of the Na,K-ATPase is located on the cytoplasmic side of the plasma membrane
AU - Lee, Kyunglim
AU - Guidotti, Guido
N1 - Funding Information:
We thank Jerry Lingrel for the cDNA for the rat α1 chain. This work was supported by NIH grant HL08893 to G. G.
PY - 1998/10/29
Y1 - 1998/10/29
N2 - In a previous study we indicated that the α subunit of the Na,K-ATPase has 4 transmembrane segments in the COOH terminal domain between residues Lys769 and Val939, and that both the NH2-terminus and the COOH-terminus are in the cytosol. However, there was insufficient information to determine whether there are more transmembrane segments between residues Val939 and the COOH-terminal Tyr1018. To investigate this question, we inserted the influenza virus hemagglutinin (HA)-epitope between Leu973 and Arg974 of the α1 chain, expressed the construct in COS-7 and HeLa cells and determined the membrane arrangement by indirect immunofluorescence. The results indicate that Leu973 is not on the extracellular surface of the plasma membrane. Thus, the α1 subunit is likely to possess only four complete transmembrane segments in the COOH terminal domain between residues Lys769 and Tyr1018.
AB - In a previous study we indicated that the α subunit of the Na,K-ATPase has 4 transmembrane segments in the COOH terminal domain between residues Lys769 and Val939, and that both the NH2-terminus and the COOH-terminus are in the cytosol. However, there was insufficient information to determine whether there are more transmembrane segments between residues Val939 and the COOH-terminal Tyr1018. To investigate this question, we inserted the influenza virus hemagglutinin (HA)-epitope between Leu973 and Arg974 of the α1 chain, expressed the construct in COS-7 and HeLa cells and determined the membrane arrangement by indirect immunofluorescence. The results indicate that Leu973 is not on the extracellular surface of the plasma membrane. Thus, the α1 subunit is likely to possess only four complete transmembrane segments in the COOH terminal domain between residues Lys769 and Tyr1018.
UR - http://www.scopus.com/inward/record.url?scp=0032578856&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1998.9534
DO - 10.1006/bbrc.1998.9534
M3 - Article
C2 - 9790971
AN - SCOPUS:0032578856
VL - 251
SP - 693
EP - 698
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -