Repression of interferon-γ-induced inducible nitric oxide synthase (iNOS) gene expression in microglia by sodium butyrate is mediated through specific inhibition of ERK signaling pathways

Jin Sun Park, Moon Sook Woo, So Young Kim, Won Ki Kim, Hee Sun Kim

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57 Scopus citations

Abstract

We have reported recently that sodium butyrate suppressed IFN-γ, but not the LPS-mediated induction of nitric oxide and TNF-α in microglia via the specific inhibition of NF-κB. In order to further determine the upstream signaling mechanism involved in the IFN-γ-specific down-regulation of iNOS by sodium butyrate in microglia, this study investigated the effect of sodium butyrate on the MAP kinase activities. Sodium butyrate significantly repressed the phosphorylation of ERK induced by IFN-γ, but had little effect on that induced by LPS. This suggests that sodium butyrate suppresses the IFN-γ-induced iNOS expression by inhibiting the ERK to NF-κB pathway. In addition, it was found that sodium butyrate suppressed the IFN-γ-induced interferon regulatory factor 1 (IRF-1) expression via the inhibition of ERK. Therefore, the ERK signaling pathway appears to play a key role in the sodium butyrate-mediated down-regulation of iNOS in the IFN-γ-stimulated microglia.

Original languageEnglish
Pages (from-to)56-64
Number of pages9
JournalJournal of Neuroimmunology
Volume168
Issue number1-2
DOIs
StatePublished - Nov 2005

Bibliographical note

Funding Information:
This work was supported by a grant to H.S.Kim (03-PJ1-PG3-21300-0047) from the Korean Health 21 R&D Project, Ministry of Health and Welfare, Republic of Korea.

Keywords

  • ERK
  • IRF-1
  • Microglia
  • NF-κB
  • Sodium butyrate
  • iNOS

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