Renoprotective effect of red ginseng in gentamicin-induced acute kidney injury

Hyun Soo Shin, Mina Yu, Mijin Kim, Hack Sun Choi, Duk Hee Kang

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43 Scopus citations


Aminoglycoside-induced nephrotoxicity is one of the prevalent causes of acute kidney injury (AKI). Oxidative stressmediated apoptosis of renal tubular cells is known to be a major mechanism of renal injury. Red ginseng extract (RGE) has been reported to possess antioxidant and immune-modulatory activities. We investigated the effect of RGE on gentamicin (GM)-induced apoptosis and oxidative stress in cultured renal tubular cells and animal model of GM-induced AKI. GM induced the generation of reactive oxygen species (ROS) with an increase in NADPH oxidase (NOX) activity and mitochondrial oxidation in NRK-52E cells that were ameliorated with RGE. GM-induced apoptosis of NRK-52E cells, which was associated with an increased expression of mitochondrial Bax, cytosolic cytochrome c, and cleaved caspase-9 and-3, along with a decrease in bcl-2 expression, was also blocked by RGE. In an animal model of GM-induced AKI, RGE treatment significantly attenuated renal dysfunction, cell apoptosis, and tubular damage. RGE ameliorated ROS production in rats with GM-induced AKI, as demonstrated by an increase in the reduced form of glutathione in renal cortex and a decrease in urinary excretion of 8-hydroxy-20-deoxyguanosine. Our results suggest that RGE protects the kidney from GM-induced AKI via the mechanism of modulation of oxidative stress.

Original languageEnglish
Pages (from-to)1147-1160
Number of pages14
JournalLaboratory Investigation
Issue number10
StatePublished - 1 Jan 2014

Bibliographical note

Funding Information:
This research was supported by the Bio & Medical Technology Development Program of the National Research Foundation (NRF) funded by the Korean government (MEST) (2010-0019866, 2012R1A2A2A01013541), the Korea Society of Ginseng (2008), the Ewha Global Top5 Grant 2013 of Ewha Womans University and Hi Seoul Science (Humanities) Fellowship funded by Seoul Scholarship Foundation.

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