Regulation of cancer cell death by a novel compound, C604, in a c-Myc-overexpressing cellular environment

Mun Jeong Jo, A. Rome Paek, Ji Seung Choi, Chang Youp Ok, Kyung Chae Jeong, Jae Hyang Lim, Seok Hyun Kim, Hye Jin You

Research output: Contribution to journalArticlepeer-review


The proto-oncogene c-Myc has been implicated in a variety of cellular processes, such as proliferation, differentiation and apoptosis. Several c-Myc targets have been studied; however, selective regulation of c-Myc is not easy in cancer cells. Herein, we attempt to identify chemical compounds that induce cell death in c-Myc-overexpressing cells (STF-cMyc and STF-Control) by conducting MTS assays on approximately 4000 chemical compounds. One compound, C604, induced cell death in STF-cMyc cells but not STF-Control cells. Apoptotic proteins, including caspase-3 and poly(ADP-ribose) polymerase (PARP), were cleaved in C604-treated STF-cMyc cells. In addition, SW620, HCT116 and NCI-H23 cells, which exhibit higher basal levels of c-Myc, underwent apoptotic cell death in response to C604, suggesting a role for C604 as an inducer of apoptosis in cancer cells with c-Myc amplification. C604 induced cell cycle arrest at the G2/M phase in cells, which was not affected by apoptotic inhibitors. Interestingly, C604 induced accumulation of c-Myc and Cdc25A proteins. In summary, a chemical compound was identified that may induce cell death in cancer cells with c-Myc amplification specifically through an apoptotic pathway.

Original languageEnglish
Pages (from-to)257-265
Number of pages9
JournalEuropean Journal of Pharmacology
StatePublished - 15 Dec 2015


  • Apoptosis
  • c-Myc
  • Cancer
  • Caspase-3


Dive into the research topics of 'Regulation of cancer cell death by a novel compound, C604, in a c-Myc-overexpressing cellular environment'. Together they form a unique fingerprint.

Cite this