Both acute myeloid leukemia 1 and c-Fos are regulatory factors of hematopoietic cell differentiation. We identified that the c-fos promoter contains an acute myeloid leukemia 1 binding site at nucleotide positions -6-+14. c-fos promoter activity was induced by transient overexpression of acute myeloid leukemia 1 in Jurkat T-cells, but not by that of the short form of acute myeloid leukemia 1-MTG8, a chimeric acute myeloid leukemia 1 protein. In 32Dcl3 myeloid cells, stable overexpression of acute myeloid leukemia 1-MTG8 blocked the c-fos gene transcription and cell differentiation, but that of acute myeloid leukemia did not. These data suggest that acute myeloid leukemia 1 and acute myeloid leukemia 1-MTG8 reciprocally regulate the myeloid cell differentiation, possibly by the way of regulating c-fos gene transcription. Copyright (C) 1999 Federation of European Biochemical Societies.
Bibliographical noteFunding Information:
The c-fos promoter/luciferase reporter gene (pFos-lcf) and pFos(SP65) were generously provided by Dr Ron Prywes, Columbia University, New York, USA and Dr Tom Kerppola, Howard Hughes Medical Institute, Ann Arbor, MI, USA, respectively. This work was supported by the Korean Science and Engineering Foundation (KOSEF).
- Acute myeloid leukemia 1
- Acute myeloid leukemia 1-MTG8
- Myeloid cell differentiation