Region specific expression of NMDA receptor NR1 subunit mRNA in hypothalamus and pons following chronic morphine treatment

Hong Zhu; Choon Gon Jang; Tangeng Ma; Seikwan Oh; Robin W. Rockhold; Ing K. Ho

doi:10.1016/S0014-2999(98)00861-9

Region specific expression of NMDA receptor NR1 subunit mRNA in hypothalamus and pons following chronic morphine treatment

Hong Zhu, Choon Gon Jang, Tangeng Ma, Seikwan Oh, Robin W. Rockhold, Ing K. Ho

Department of Molecular Medicine

Research output: Contribution to journal › Article › peer-review

52 Scopus citations

Abstract

The NMDA receptor has been implicated in opioid tolerance and physical dependence. Using in situ hybridization techniques, the effects of chronic morphine treatment on the expression of mRNAs encoding the NMDA receptor subunits NR1, NR2A, and NR2B were investigated. A significant increase in the level of the NR1 subunit mRNA was found in the locus coeruleus and the hypothalamic paraventricular nucleus following 3 days of intracerebroventricular (i.c.v.) morphine infusion (26 nmol μl^-1 h^-1) through osmotic minipumps. No changes were detected in expression of the NR1 mRNA in the frontal cortex, caudate-putamen, nucleus accumbens, amygdala, CA1, CA2, and the dentate gyrus of the hippocampus, and in the central grey after morphine treatment. The expression of NR2A and NR2B subunit mRNAs did not change after morphine treatment in any brain region. These results suggest that changes in gene expression of the NR1 subunit of the NMDA receptor are involved in the development of morphine tolerance and dependence. Copyright (C) 1999 Elsevier Science B.V.

Original language	English
Pages (from-to)	47-54
Number of pages	8
Journal	European Journal of Pharmacology
Volume	365
Issue number	1
DOIs	https://doi.org/10.1016/S0014-2999(98)00861-9
State	Published - 15 Jan 1999

Bibliographical note

Funding Information:
We thank Dr. Zhi He for her technical assistance in anesthesia. This work was supported by Grant DA 05828 from the National Institute on Drug Abuse.

Keywords

In situ hybridization
Morphine
NMDA receptor
Physical dependence
Tolerance

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title = "Region specific expression of NMDA receptor NR1 subunit mRNA in hypothalamus and pons following chronic morphine treatment",

abstract = "The NMDA receptor has been implicated in opioid tolerance and physical dependence. Using in situ hybridization techniques, the effects of chronic morphine treatment on the expression of mRNAs encoding the NMDA receptor subunits NR1, NR2A, and NR2B were investigated. A significant increase in the level of the NR1 subunit mRNA was found in the locus coeruleus and the hypothalamic paraventricular nucleus following 3 days of intracerebroventricular (i.c.v.) morphine infusion (26 nmol μl-1 h-1) through osmotic minipumps. No changes were detected in expression of the NR1 mRNA in the frontal cortex, caudate-putamen, nucleus accumbens, amygdala, CA1, CA2, and the dentate gyrus of the hippocampus, and in the central grey after morphine treatment. The expression of NR2A and NR2B subunit mRNAs did not change after morphine treatment in any brain region. These results suggest that changes in gene expression of the NR1 subunit of the NMDA receptor are involved in the development of morphine tolerance and dependence. Copyright (C) 1999 Elsevier Science B.V.",

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author = "Hong Zhu and Jang, {Choon Gon} and Tangeng Ma and Seikwan Oh and Rockhold, {Robin W.} and Ho, {Ing K.}",

note = "Funding Information: We thank Dr. Zhi He for her technical assistance in anesthesia. This work was supported by Grant DA 05828 from the National Institute on Drug Abuse.",

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AU - Zhu, Hong

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AU - Ma, Tangeng

AU - Oh, Seikwan

AU - Rockhold, Robin W.

AU - Ho, Ing K.

N1 - Funding Information: We thank Dr. Zhi He for her technical assistance in anesthesia. This work was supported by Grant DA 05828 from the National Institute on Drug Abuse.

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Y1 - 1999/1/15

N2 - The NMDA receptor has been implicated in opioid tolerance and physical dependence. Using in situ hybridization techniques, the effects of chronic morphine treatment on the expression of mRNAs encoding the NMDA receptor subunits NR1, NR2A, and NR2B were investigated. A significant increase in the level of the NR1 subunit mRNA was found in the locus coeruleus and the hypothalamic paraventricular nucleus following 3 days of intracerebroventricular (i.c.v.) morphine infusion (26 nmol μl-1 h-1) through osmotic minipumps. No changes were detected in expression of the NR1 mRNA in the frontal cortex, caudate-putamen, nucleus accumbens, amygdala, CA1, CA2, and the dentate gyrus of the hippocampus, and in the central grey after morphine treatment. The expression of NR2A and NR2B subunit mRNAs did not change after morphine treatment in any brain region. These results suggest that changes in gene expression of the NR1 subunit of the NMDA receptor are involved in the development of morphine tolerance and dependence. Copyright (C) 1999 Elsevier Science B.V.

AB - The NMDA receptor has been implicated in opioid tolerance and physical dependence. Using in situ hybridization techniques, the effects of chronic morphine treatment on the expression of mRNAs encoding the NMDA receptor subunits NR1, NR2A, and NR2B were investigated. A significant increase in the level of the NR1 subunit mRNA was found in the locus coeruleus and the hypothalamic paraventricular nucleus following 3 days of intracerebroventricular (i.c.v.) morphine infusion (26 nmol μl-1 h-1) through osmotic minipumps. No changes were detected in expression of the NR1 mRNA in the frontal cortex, caudate-putamen, nucleus accumbens, amygdala, CA1, CA2, and the dentate gyrus of the hippocampus, and in the central grey after morphine treatment. The expression of NR2A and NR2B subunit mRNAs did not change after morphine treatment in any brain region. These results suggest that changes in gene expression of the NR1 subunit of the NMDA receptor are involved in the development of morphine tolerance and dependence. Copyright (C) 1999 Elsevier Science B.V.

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KW - Morphine

KW - NMDA receptor

KW - Physical dependence

KW - Tolerance

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Region specific expression of NMDA receptor NR1 subunit mRNA in hypothalamus and pons following chronic morphine treatment

Abstract

Bibliographical note

Keywords

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