Reduction of perchlorate by salt tolerant bacterial consortia

Hee Wook Ryu; Seong Jin Nor; Kyung Eun Moon; Kyung Suk Cho; Daniel K. Cha; Kang In Rhee

doi:10.1016/j.biortech.2011.09.115

Reduction of perchlorate by salt tolerant bacterial consortia

Hee Wook Ryu, Seong Jin Nor, Kyung Eun Moon, Kyung Suk Cho, Daniel K. Cha, Kang In Rhee

Department of Environmental Science & Engineering

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Two perchlorate-reducing bacterial consortia (PRBC) were obtained by enrichment cultures from polluted marine sediments. Non-salt-tolerant PRBC (N-PRBC) was enriched without the addition of NaCl, and salt tolerant-PRBC (ST-PRBC) was enriched with 30g-NaClL ^-1. Although the perchlorate reduction rates decreased with increasing NaCl concentration, ST-PRBC (resp., N-PRBC) could reduce perchlorate until 75g-NaClL ^-1 (resp., 30g-NaClL ^-1). The reduction yield (1.34±0.05mg-perchlorate per mg-acetate) and maximum perchlorate reduction rate (86mg-perchlorateL ^-1h ^-1) of ST-PRBC was higher than those (1.16±0.03mg-perchlorate per mg-acetate and 48mg-perchlorateL ^-1h ^-1) of N-PRBC. Kinetic analysis showed that NaCl acted as an uncompetitive inhibitor against both PRBCs. The inhibition constants were 25 and 41mg-NaClL ^-1 for N-PRBC and ST-PRBC, respectively.

Original language	English
Pages (from-to)	279-285
Number of pages	7
Journal	Bioresource Technology
Volume	103
Issue number	1
DOIs	https://doi.org/10.1016/j.biortech.2011.09.115
State	Published - Jan 2012

Bibliographical note

Funding Information:
This work was supported by the Basic Research Program through an NRF grant (No. 2009-0075052 ) and the Resource Recycling R&D Center of the 21C Frontier R&D Program funded by the Ministry of Education, Science, and Technology (MEST).

Keywords

Perchlorate-reducing bacteria
Salt tolerance
Uncompetitive inhibition

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10.1016/j.biortech.2011.09.115

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title = "Reduction of perchlorate by salt tolerant bacterial consortia",

abstract = "Two perchlorate-reducing bacterial consortia (PRBC) were obtained by enrichment cultures from polluted marine sediments. Non-salt-tolerant PRBC (N-PRBC) was enriched without the addition of NaCl, and salt tolerant-PRBC (ST-PRBC) was enriched with 30g-NaClL -1. Although the perchlorate reduction rates decreased with increasing NaCl concentration, ST-PRBC (resp., N-PRBC) could reduce perchlorate until 75g-NaClL -1 (resp., 30g-NaClL -1). The reduction yield (1.34±0.05mg-perchlorate per mg-acetate) and maximum perchlorate reduction rate (86mg-perchlorateL -1h -1) of ST-PRBC was higher than those (1.16±0.03mg-perchlorate per mg-acetate and 48mg-perchlorateL -1h -1) of N-PRBC. Kinetic analysis showed that NaCl acted as an uncompetitive inhibitor against both PRBCs. The inhibition constants were 25 and 41mg-NaClL -1 for N-PRBC and ST-PRBC, respectively.",

keywords = "Perchlorate-reducing bacteria, Salt tolerance, Uncompetitive inhibition",

author = "Ryu, {Hee Wook} and Nor, {Seong Jin} and Moon, {Kyung Eun} and Cho, {Kyung Suk} and Cha, {Daniel K.} and Rhee, {Kang In}",

note = "Funding Information: This work was supported by the Basic Research Program through an NRF grant (No. 2009-0075052 ) and the Resource Recycling R&D Center of the 21C Frontier R&D Program funded by the Ministry of Education, Science, and Technology (MEST).",

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AU - Nor, Seong Jin

AU - Moon, Kyung Eun

AU - Cho, Kyung Suk

AU - Cha, Daniel K.

AU - Rhee, Kang In

N1 - Funding Information: This work was supported by the Basic Research Program through an NRF grant (No. 2009-0075052 ) and the Resource Recycling R&D Center of the 21C Frontier R&D Program funded by the Ministry of Education, Science, and Technology (MEST).

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N2 - Two perchlorate-reducing bacterial consortia (PRBC) were obtained by enrichment cultures from polluted marine sediments. Non-salt-tolerant PRBC (N-PRBC) was enriched without the addition of NaCl, and salt tolerant-PRBC (ST-PRBC) was enriched with 30g-NaClL -1. Although the perchlorate reduction rates decreased with increasing NaCl concentration, ST-PRBC (resp., N-PRBC) could reduce perchlorate until 75g-NaClL -1 (resp., 30g-NaClL -1). The reduction yield (1.34±0.05mg-perchlorate per mg-acetate) and maximum perchlorate reduction rate (86mg-perchlorateL -1h -1) of ST-PRBC was higher than those (1.16±0.03mg-perchlorate per mg-acetate and 48mg-perchlorateL -1h -1) of N-PRBC. Kinetic analysis showed that NaCl acted as an uncompetitive inhibitor against both PRBCs. The inhibition constants were 25 and 41mg-NaClL -1 for N-PRBC and ST-PRBC, respectively.

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Reduction of perchlorate by salt tolerant bacterial consortia

Abstract

Bibliographical note

Keywords

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