Two perchlorate-reducing bacterial consortia (PRBC) were obtained by enrichment cultures from polluted marine sediments. Non-salt-tolerant PRBC (N-PRBC) was enriched without the addition of NaCl, and salt tolerant-PRBC (ST-PRBC) was enriched with 30g-NaClL -1. Although the perchlorate reduction rates decreased with increasing NaCl concentration, ST-PRBC (resp., N-PRBC) could reduce perchlorate until 75g-NaClL -1 (resp., 30g-NaClL -1). The reduction yield (1.34±0.05mg-perchlorate per mg-acetate) and maximum perchlorate reduction rate (86mg-perchlorateL -1h -1) of ST-PRBC was higher than those (1.16±0.03mg-perchlorate per mg-acetate and 48mg-perchlorateL -1h -1) of N-PRBC. Kinetic analysis showed that NaCl acted as an uncompetitive inhibitor against both PRBCs. The inhibition constants were 25 and 41mg-NaClL -1 for N-PRBC and ST-PRBC, respectively.
Bibliographical noteFunding Information:
This work was supported by the Basic Research Program through an NRF grant (No. 2009-0075052 ) and the Resource Recycling R&D Center of the 21C Frontier R&D Program funded by the Ministry of Education, Science, and Technology (MEST).
- Perchlorate-reducing bacteria
- Salt tolerance
- Uncompetitive inhibition