Reduction of FBS concentration through adaptation process in mammalian cell culture and addition of silkworm hemolymph in insect cell culture

Animal cell culture media are usually supplemented with fetal bovine serum (FBS); however, the use of FBS presents certain problems including high cost. By using an adaptation process and the addition of silkworm hemolymph, the FBS concentration can be reduced without causing a significant decrease in cell growth.