Reduced Graphene Oxide-Oligonucleotide Interfaces: Understanding Based on Electrochemical Oxidation of Guanines

Anjong Florence Tikum, Jeong Won Ko, Soojin Kim, Jinheung Kim

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Investigation into the interactions between biomolecules DNA/RNA and carbon nanomaterials is very important for applications in bioassays and bioanalysis. Graphene and graphene oxide (GO) have been successfully adopted by exploiting the binding affinity difference between single-stranded oligonucleotides (ssDNA) and double-stranded oligonucleotides (dsDNA) to graphene sheets. In this work, we describe the electrochemical DNA oxidation with [Ru(bpy)3]2+ to understand the interaction between dsDNA (and corresponding ssDNA) and reduced graphene oxide (rGO). The electrochemical oxidation rate of guanine bases of ssDNA bound to rGO by electrochemically generated [Ru(bpy)3]3+ was much slower than those unbound to rGO. Our study revealed that ssDNA constrained on rGO was significantly protected from the electron transfer to [Ru(bpy)3]3+ because of π, π-stacking interaction between nucleobases and rGO. On the other hand, the oxidation rates of 11-, 20-, and 27-mer dsDNA bound to rGO increased relative to those of dsDNA alone, demonstrating that the guanine bases of dsDNA on the interaction with rGO became more accessible to [Ru(bpy)3]3+. Our electrochemical data illustrated that dsDNA could be totally or partially dehybridized and bind to rGO to form ssDNA/rGO. Furthermore, absorption, circular dichroism spectra, and fluorescence measurements of ethidium bromide using ssDNA and dsDNA with rGO supported the dehybridization of dsDNA in the presence of rGO.

Original languageEnglish
Pages (from-to)15464-15470
Number of pages7
JournalACS Omega
Volume3
Issue number11
DOIs
StatePublished - 14 Nov 2018

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation (NRF) grant funded by the Korean government (NRF-2017R1A5A1015365 and NRF-2016R1A2B4012488) and “Next Generation Carbon Upcycling Project” (Project No. 2017M1A2A2042517) through the NRF funded by the Ministry of Science and ICT.

Publisher Copyright:
© 2018 American Chemical Society.

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