Red2Flpe-SCON: a versatile, multicolor strategy for generating mosaic conditional knockout mice

Szu Hsien Sam Wu, Somi Kim, Heetak Lee, Ji Hyun Lee, So Yeon Park, Réka Bakonyi, Isaree Teriyapirom, Natalia Hallay, Sandra Pilat-Carotta, Hans Christian Theussl, Jihoon Kim, Joo Hyeon Lee, Benjamin D. Simons, Jong Kyoung Kim, Gabriele Colozza, Bon Kyoung Koo

Research output: Contribution to journalArticlepeer-review

Abstract

Image-based lineage tracing enables tissue turnover kinetics and lineage potentials of different adult cell populations to be investigated. Previously, we reported a genetic mouse model system, Red2Onco, which ectopically expressed mutated oncogenes together with red fluorescent proteins (RFP). This system enabled the expansion kinetics and neighboring effects of oncogenic clones to be dissected. We now report Red2Flpe-SCON: a mosaic knockout system that uses multicolor reporters to label both mutant and wild-type cells. We develop the Red2Flpe mouse line for red clone-specific Flpe expression, as well as the FRT-based SCON (Short Conditional IntrON) method to facilitate tunable conditional mosaic knockouts in mice. We use the Red2Flpe-SCON method to study Sox2 mutant clonal analysis in the esophageal epithelium of adult mice which reveal that the stem cell gene, Sox2, is less essential for adult stem cell maintenance itself, but rather for stem cell proliferation and differentiation.

Original languageEnglish
Article number4963
JournalNature Communications
Volume15
Issue number1
DOIs
StatePublished - Dec 2024

Bibliographical note

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© The Author(s) 2024.

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