Recovery of poly(3-hydroxybutyrate) from high cell density culture of Ralstonia eutropha by direct addition of sodium dodecyl sulfate

Mia Kim, Kyung Suk Cho, Hee Wook Ryu, Eun Gyo Lee, Yong Keun Chang

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

A simple and effective method for the recovery poly(3-hydroxybutyrate) [P(3HB)] directly from high cell density culture broth with no pretreatment steps has been developed. This method consists of direct addition of sodium dodecyl sulfate (SDS) to the culture broth, shaking, heat treatment, and washing steps. When the SDS/biomass ratio was higher than 0.4, the purity of recovered P(3HB) was over 95% for various cell concentrations of 50-300 g dry cell 1-1, with the highest value of 97%. The recovery of P(3HB) was over 90% regardless of cell concentration and SDS dosage (SDS/biomass ratios, 0.1-0.7). One g SDS digests 0.72 g non-P(3HB) cell materials. The reduction in molecular weight, due to degradation of P(3HB) by SDS, was negligible.

Original languageEnglish
Pages (from-to)55-59
Number of pages5
JournalBiotechnology Letters
Volume25
Issue number1
DOIs
StatePublished - Jan 2003

Keywords

  • High cell density culture
  • Poly(3-hydroxybutyrate)
  • Ralstonia eutropha
  • Recovery
  • Sodium dodecyl sulfate

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