TY - JOUR
T1 - Recent developments and applications of capillary electrophoresis with laser-induced fluorescence detection in biological samples
AU - Ban, Eunmi
AU - Song, Eun Joo
N1 - Funding Information:
We gratefully acknowledge the support of GRL Program ( 20110021713 ) from the National Research Foundation of Korea and an institutional grant from Korean Institute of Science and Technology ( 2E23810 and 2E24080 ).
PY - 2013/6/5
Y1 - 2013/6/5
N2 - Since the human genomic project, the analysis of biomolecules using biotechnology and biopharmaceuticals has become increasingly important for the investigation of molecular mechanisms of biological processes and disease, as well as for the discovery of new biomarkers and drug targets. Among a number of analytical tools and technologies, capillary electrophoresis (CE) is emerging as a useful analytical tool for analyzing various biomolecules, as well as for performing genome sequencing in biological studies because of the simple sample preparation involved in the method and the efficient resolution of compounds of various sizes. In particular, CE with laser-induced fluorescence (CE-LIF) detection is a promising and relatively rapidly developing tool with the potential to provide a highly sensitive and specific analysis of biological molecules. This paper gives a short overview of the methodological and instrumental developments and applications of microchip-based CE and CE-LIF for the analysis of DNA, RNA, proteins, and peptides in the past 3 years.
AB - Since the human genomic project, the analysis of biomolecules using biotechnology and biopharmaceuticals has become increasingly important for the investigation of molecular mechanisms of biological processes and disease, as well as for the discovery of new biomarkers and drug targets. Among a number of analytical tools and technologies, capillary electrophoresis (CE) is emerging as a useful analytical tool for analyzing various biomolecules, as well as for performing genome sequencing in biological studies because of the simple sample preparation involved in the method and the efficient resolution of compounds of various sizes. In particular, CE with laser-induced fluorescence (CE-LIF) detection is a promising and relatively rapidly developing tool with the potential to provide a highly sensitive and specific analysis of biological molecules. This paper gives a short overview of the methodological and instrumental developments and applications of microchip-based CE and CE-LIF for the analysis of DNA, RNA, proteins, and peptides in the past 3 years.
KW - Biomolecules
KW - Capillary electrophoresis
KW - Laser-induced fluorescence
UR - http://www.scopus.com/inward/record.url?scp=84878218184&partnerID=8YFLogxK
U2 - 10.1016/j.jchromb.2013.04.028
DO - 10.1016/j.jchromb.2013.04.028
M3 - Review article
C2 - 23685428
AN - SCOPUS:84878218184
SN - 1570-0232
VL - 929
SP - 180
EP - 186
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
ER -