TY - JOUR
T1 - Recent advances in enzyme-activated NIR fluorescent probes for biological applications
AU - Jeong, Hyunsun
AU - Wu, Xiaofeng
AU - Lee, Jun Seok
AU - Yoon, Juyoung
N1 - Publisher Copyright:
© 2023 Elsevier B.V.
PY - 2023/11
Y1 - 2023/11
N2 - Enzymes and their regulations are employed as indicators for various diseases, including cancer. To directly analyze the activity of the enzymes within a biological system, enzyme-activated near-infrared (NIR) fluorescent probes are being continuously developed. These probes enable clear imaging of enzyme activity in real time with high spatiotemporal resolution, while preserving their unique advantages such as high sensitivity, reduced autofluorescence, and low light scattering. Enzyme expression levels can also be investigated by evaluating the emission intensity. Owing to these strengths, enzyme-activated NIR fluorescent probes have been applied in various clinical areas such as biomedical imaging, quantitative analysis, and surgery. Therefore, the development of NIR fluorescent probes that could be specifically activated by disease-related enzymes is a crucial task. In this review, we introduce several enzymes associated with disorders and summarize the relevant activated NIR fluorescent probes reported from 2020 to 2023.
AB - Enzymes and their regulations are employed as indicators for various diseases, including cancer. To directly analyze the activity of the enzymes within a biological system, enzyme-activated near-infrared (NIR) fluorescent probes are being continuously developed. These probes enable clear imaging of enzyme activity in real time with high spatiotemporal resolution, while preserving their unique advantages such as high sensitivity, reduced autofluorescence, and low light scattering. Enzyme expression levels can also be investigated by evaluating the emission intensity. Owing to these strengths, enzyme-activated NIR fluorescent probes have been applied in various clinical areas such as biomedical imaging, quantitative analysis, and surgery. Therefore, the development of NIR fluorescent probes that could be specifically activated by disease-related enzymes is a crucial task. In this review, we introduce several enzymes associated with disorders and summarize the relevant activated NIR fluorescent probes reported from 2020 to 2023.
KW - Activatable probe
KW - Bioapplication
KW - Enzyme
KW - Fluorescence imaging
KW - NIR emission
UR - http://www.scopus.com/inward/record.url?scp=85173619646&partnerID=8YFLogxK
U2 - 10.1016/j.trac.2023.117335
DO - 10.1016/j.trac.2023.117335
M3 - Review article
AN - SCOPUS:85173619646
SN - 0165-9936
VL - 168
JO - TrAC - Trends in Analytical Chemistry
JF - TrAC - Trends in Analytical Chemistry
M1 - 117335
ER -
