Real-time tracking of highly luminescent mesoporous silica particles modified with europium β-diketone chelates in living cells

Jong Seok Kim; Sung Ki Lee; Hansol Doh; Myeong Yun Kim; Do Kyung Kim

doi:10.3390/nano11020343

Real-time tracking of highly luminescent mesoporous silica particles modified with europium β-diketone chelates in living cells

Jong Seok Kim, Sung Ki Lee, Hansol Doh, Myeong Yun Kim, Do Kyung Kim

Department of Food Science & Biotechnology

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Highly luminescent europium complexes modified mesoporous silica particles (MSP) were synthesized as an imaging probes for both in-vitro diagnostic and in-vivo cellular tracking agents. Europium β-diketone chelates (4,4,4-trifluoro-l-(2-thienyl)-l,3-butanedione) trioctylphosphine europium (III) (Eu(TTA)3(P(Oct)3)3) were incorporated inside the nanocavities that existed in hierarchical MSP (Eu@MSP). The MSP and Eu@MSP on mouse bone marrow-derived macrophages (BMDMs) did not show any toxic effect. The MSP and Eu@MSP in the BMDMs were found at cytoplasm without any degradation and immunogenicity. However, both pro-and antiinflammatory cytokines of macrophages were significantly increased when lipopolysaccharide and a high concentration (100 μg/mL) of MSP and Eu@MSP were treated simultaneously.

Original language	English
Article number	343
Pages (from-to)	1-16
Number of pages	16
Journal	Nanomaterials
Volume	11
Issue number	2
DOIs	https://doi.org/10.3390/nano11020343
State	Published - Feb 2021

Bibliographical note

Funding Information:
Funding: This research was supported by Basic Science Research Program through the Na-tional Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (NRF-2017R1A2B4005167) and the Ministry of Education (NRF-2018R1C1B6007421).

Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Keywords

Beta-diketone
Europium complex
Inflammation
Live cell image
Mesoporous silica

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title = "Real-time tracking of highly luminescent mesoporous silica particles modified with europium β-diketone chelates in living cells",

abstract = "Highly luminescent europium complexes modified mesoporous silica particles (MSP) were synthesized as an imaging probes for both in-vitro diagnostic and in-vivo cellular tracking agents. Europium β-diketone chelates (4,4,4-trifluoro-l-(2-thienyl)-l,3-butanedione) trioctylphosphine europium (III) (Eu(TTA)3(P(Oct)3)3) were incorporated inside the nanocavities that existed in hierarchical MSP (Eu@MSP). The MSP and Eu@MSP on mouse bone marrow-derived macrophages (BMDMs) did not show any toxic effect. The MSP and Eu@MSP in the BMDMs were found at cytoplasm without any degradation and immunogenicity. However, both pro-and antiinflammatory cytokines of macrophages were significantly increased when lipopolysaccharide and a high concentration (100 μg/mL) of MSP and Eu@MSP were treated simultaneously.",

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author = "Kim, {Jong Seok} and Lee, {Sung Ki} and Hansol Doh and Kim, {Myeong Yun} and Kim, {Do Kyung}",

note = "Funding Information: Funding: This research was supported by Basic Science Research Program through the Na-tional Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (NRF-2017R1A2B4005167) and the Ministry of Education (NRF-2018R1C1B6007421). Publisher Copyright: {\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland.",

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AU - Kim, Jong Seok

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AU - Doh, Hansol

AU - Kim, Myeong Yun

AU - Kim, Do Kyung

N1 - Funding Information: Funding: This research was supported by Basic Science Research Program through the Na-tional Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (NRF-2017R1A2B4005167) and the Ministry of Education (NRF-2018R1C1B6007421). Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

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N2 - Highly luminescent europium complexes modified mesoporous silica particles (MSP) were synthesized as an imaging probes for both in-vitro diagnostic and in-vivo cellular tracking agents. Europium β-diketone chelates (4,4,4-trifluoro-l-(2-thienyl)-l,3-butanedione) trioctylphosphine europium (III) (Eu(TTA)3(P(Oct)3)3) were incorporated inside the nanocavities that existed in hierarchical MSP (Eu@MSP). The MSP and Eu@MSP on mouse bone marrow-derived macrophages (BMDMs) did not show any toxic effect. The MSP and Eu@MSP in the BMDMs were found at cytoplasm without any degradation and immunogenicity. However, both pro-and antiinflammatory cytokines of macrophages were significantly increased when lipopolysaccharide and a high concentration (100 μg/mL) of MSP and Eu@MSP were treated simultaneously.

AB - Highly luminescent europium complexes modified mesoporous silica particles (MSP) were synthesized as an imaging probes for both in-vitro diagnostic and in-vivo cellular tracking agents. Europium β-diketone chelates (4,4,4-trifluoro-l-(2-thienyl)-l,3-butanedione) trioctylphosphine europium (III) (Eu(TTA)3(P(Oct)3)3) were incorporated inside the nanocavities that existed in hierarchical MSP (Eu@MSP). The MSP and Eu@MSP on mouse bone marrow-derived macrophages (BMDMs) did not show any toxic effect. The MSP and Eu@MSP in the BMDMs were found at cytoplasm without any degradation and immunogenicity. However, both pro-and antiinflammatory cytokines of macrophages were significantly increased when lipopolysaccharide and a high concentration (100 μg/mL) of MSP and Eu@MSP were treated simultaneously.

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Real-time tracking of highly luminescent mesoporous silica particles modified with europium β-diketone chelates in living cells

Abstract

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Keywords

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