Real-time Selective Detection of Hydrogen Peroxide Based on a Tantalum Deposited Pencil Lead Electrode for Evaluation of Enzyme Activities

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Abstract

In this study, we have carried out electrodeposition of tantalum (Ta) nanostructures on pencil lead electrode in non-aqueous media at room temperature by applying a constant potential. The deposited Ta on pencil lead was examined for the catalytic effect regarding hydrogen peroxide (H2O2) reduction with voltammetry and amperometry. Ta/pencil lead electrode exhibited amperometric sensitivity of 0.317 μA mM−1 cm−2 and fast response time of 0.75 s, where selective detection of H2O2 was fulfilled without interruption from common electroactive biomaterials such as O2, uric acid, ascorbic acid, dopamine, acetamidophenol, and glucose. For practical applications, the dynamic concentration changes of H2O2 during catalase and glucose oxidase-involved reactions, either eliminating or producing H2O2, were successfully traced in real time with as-prepared electrode. From the kinetics study for catalase and glucose oxidase, we evaluated Michaelis constants (Km app) as 7.8 mM for catalase and 37 mM for glucose oxidase, respectively.

Original languageEnglish
Pages (from-to)2254-2260
Number of pages7
JournalElectroanalysis
Volume29
Issue number10
DOIs
StatePublished - Oct 2017

Bibliographical note

Funding Information:
This work was financially supported by the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and Future Planning (2017R1A2B4002159 for CL) and (2017R1A2A2A14001137 for YL).

Publisher Copyright:
© 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim

Keywords

  • Michaelis constant
  • Tantalum (Ta) electrodeposited pencil lead
  • catalase
  • electrochemical hydrogen peroxide sensing
  • glucose oxidase

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