Real-time Selective Detection of Hydrogen Peroxide Based on a Tantalum Deposited Pencil Lead Electrode for Evaluation of Enzyme Activities

Ara Jo; Youngmi Lee; Chongmok Lee

doi:10.1002/elan.201700315

Real-time Selective Detection of Hydrogen Peroxide Based on a Tantalum Deposited Pencil Lead Electrode for Evaluation of Enzyme Activities

Ara Jo, Youngmi Lee, Chongmok Lee

Department of Chemistry & Nanoscience

Research output: Contribution to journal › Article › peer-review

Abstract

In this study, we have carried out electrodeposition of tantalum (Ta) nanostructures on pencil lead electrode in non-aqueous media at room temperature by applying a constant potential. The deposited Ta on pencil lead was examined for the catalytic effect regarding hydrogen peroxide (H₂O₂) reduction with voltammetry and amperometry. Ta/pencil lead electrode exhibited amperometric sensitivity of 0.317 μA mM⁻¹ cm⁻² and fast response time of 0.75 s, where selective detection of H₂O₂ was fulfilled without interruption from common electroactive biomaterials such as O₂, uric acid, ascorbic acid, dopamine, acetamidophenol, and glucose. For practical applications, the dynamic concentration changes of H₂O₂ during catalase and glucose oxidase-involved reactions, either eliminating or producing H₂O₂, were successfully traced in real time with as-prepared electrode. From the kinetics study for catalase and glucose oxidase, we evaluated Michaelis constants (K_m ^app) as 7.8 mM for catalase and 37 mM for glucose oxidase, respectively.

Original language	English
Pages (from-to)	2254-2260
Number of pages	7
Journal	Electroanalysis
Volume	29
Issue number	10
DOIs	https://doi.org/10.1002/elan.201700315
State	Published - Oct 2017

Bibliographical note

Funding Information:
This work was financially supported by the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and Future Planning (2017R1A2B4002159 for CL) and (2017R1A2A2A14001137 for YL).

Publisher Copyright:
© 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim

Keywords

Michaelis constant
Tantalum (Ta) electrodeposited pencil lead
catalase
electrochemical hydrogen peroxide sensing
glucose oxidase

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title = "Real-time Selective Detection of Hydrogen Peroxide Based on a Tantalum Deposited Pencil Lead Electrode for Evaluation of Enzyme Activities",

abstract = "In this study, we have carried out electrodeposition of tantalum (Ta) nanostructures on pencil lead electrode in non-aqueous media at room temperature by applying a constant potential. The deposited Ta on pencil lead was examined for the catalytic effect regarding hydrogen peroxide (H2O2) reduction with voltammetry and amperometry. Ta/pencil lead electrode exhibited amperometric sensitivity of 0.317 μA mM−1 cm−2 and fast response time of 0.75 s, where selective detection of H2O2 was fulfilled without interruption from common electroactive biomaterials such as O2, uric acid, ascorbic acid, dopamine, acetamidophenol, and glucose. For practical applications, the dynamic concentration changes of H2O2 during catalase and glucose oxidase-involved reactions, either eliminating or producing H2O2, were successfully traced in real time with as-prepared electrode. From the kinetics study for catalase and glucose oxidase, we evaluated Michaelis constants (Km app) as 7.8 mM for catalase and 37 mM for glucose oxidase, respectively.",

keywords = "Michaelis constant, Tantalum (Ta) electrodeposited pencil lead, catalase, electrochemical hydrogen peroxide sensing, glucose oxidase",

author = "Ara Jo and Youngmi Lee and Chongmok Lee",

note = "Funding Information: This work was financially supported by the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and Future Planning (2017R1A2B4002159 for CL) and (2017R1A2A2A14001137 for YL). Publisher Copyright: {\textcopyright} 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim",

year = "2017",

month = oct,

doi = "10.1002/elan.201700315",

language = "English",

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AU - Jo, Ara

AU - Lee, Youngmi

AU - Lee, Chongmok

N1 - Funding Information: This work was financially supported by the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and Future Planning (2017R1A2B4002159 for CL) and (2017R1A2A2A14001137 for YL). Publisher Copyright: © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim

PY - 2017/10

Y1 - 2017/10

N2 - In this study, we have carried out electrodeposition of tantalum (Ta) nanostructures on pencil lead electrode in non-aqueous media at room temperature by applying a constant potential. The deposited Ta on pencil lead was examined for the catalytic effect regarding hydrogen peroxide (H2O2) reduction with voltammetry and amperometry. Ta/pencil lead electrode exhibited amperometric sensitivity of 0.317 μA mM−1 cm−2 and fast response time of 0.75 s, where selective detection of H2O2 was fulfilled without interruption from common electroactive biomaterials such as O2, uric acid, ascorbic acid, dopamine, acetamidophenol, and glucose. For practical applications, the dynamic concentration changes of H2O2 during catalase and glucose oxidase-involved reactions, either eliminating or producing H2O2, were successfully traced in real time with as-prepared electrode. From the kinetics study for catalase and glucose oxidase, we evaluated Michaelis constants (Km app) as 7.8 mM for catalase and 37 mM for glucose oxidase, respectively.

AB - In this study, we have carried out electrodeposition of tantalum (Ta) nanostructures on pencil lead electrode in non-aqueous media at room temperature by applying a constant potential. The deposited Ta on pencil lead was examined for the catalytic effect regarding hydrogen peroxide (H2O2) reduction with voltammetry and amperometry. Ta/pencil lead electrode exhibited amperometric sensitivity of 0.317 μA mM−1 cm−2 and fast response time of 0.75 s, where selective detection of H2O2 was fulfilled without interruption from common electroactive biomaterials such as O2, uric acid, ascorbic acid, dopamine, acetamidophenol, and glucose. For practical applications, the dynamic concentration changes of H2O2 during catalase and glucose oxidase-involved reactions, either eliminating or producing H2O2, were successfully traced in real time with as-prepared electrode. From the kinetics study for catalase and glucose oxidase, we evaluated Michaelis constants (Km app) as 7.8 mM for catalase and 37 mM for glucose oxidase, respectively.

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KW - electrochemical hydrogen peroxide sensing

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ER -

Real-time Selective Detection of Hydrogen Peroxide Based on a Tantalum Deposited Pencil Lead Electrode for Evaluation of Enzyme Activities

Abstract

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Keywords

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