Surface plasmon resonance (SPR) is a powerful technique for measuring molecular interaction in real-time. SPR can be used to detect molecule to cell interactions as well as molecule to molecule interactions. In this study, the SPR-based biosensing technique was applied to real-time monitoring of odorant-induced cellular reactions. An olfactory receptor, OR I7, was fused with a rho-tag import sequence at the N-terminus of OR I7, and expressed on the surface of human embryonic kidney (HEK)-293 cells. These cells were then immobilized on a SPR sensor chip. The intensity of the SPR response was linearly dependent on the amount of injected odorant. Among all the aldehyde containing odorants tested, the SPR response was specifically high for octanal, which is the known cognate odorant for the OR I7. This SPR response is believed to have resulted from intracellular signaling triggered by the binding of odorant molecules to the olfactory receptors expressed on the cell surface. This SPR system combined with olfactory receptor-expressed cells provides a new olfactory biosensor system for selective and quantitative detection of volatile compounds.
Bibliographical noteFunding Information:
This work was supported by the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government (MEST) (No. 2009-0080242). The authors wish to acknowledge Dr. Randall R. Reed (Department of Neurosceince, Johns Hopkins University, School of Medicine, Baltimore, Maryland, U.S.A.) for the rho-tag gene; Akihito Yasuoka (Bio-oriented Technology Reseach Advancement Institution, Saitama, Japan) for the pVL-I7 and Paul A. Gargrave (J. Hillis Miller Health Center, Department of Ophthalmology, University of Florida College of Medicine, Gainesville, Florida, U.S.A.) for the B6-30 cell line.
- Human embryonic kidney (HEK)-293 cell
- Olfactory biosensor
- Olfactory receptor I7
- Surface plasmon resonance