A bioelectronic nose for the real-time assessment of water quality was constructed with human olfactory receptor (hOR) and single-walled carbon nanotube field-effect transistor (swCNT-FET). Geosmin (GSM) and 2-methylisoborneol (MIB), mainly produced by bacteria, are representative odor compounds and also indicators of contamination in the water supply system. For the screening of hORs which respond to these compounds, we performed CRE-luciferase assays of the two odorants in heterologous cell system. Human OR51S1 for GSM and OR3A4 for MIB were selected, and nanovesicles expressing the hORs on surface were produced from HEK-293 cell. Carbon nanotube field-effect transistor was functionalized with the nanovesicles. The bioelectronic nose was able to selectively detect GSM and MIB at concentrations as low as a 10ngL-1. Furthermore, detection of these compounds from the real samples such as tap water, bottled water and river water was available without any pretreatment processes.
Bibliographical noteFunding Information:
This work was supported by the Center for Integrated Smart Sensors funded by the Ministry of Science, ICT & Future Planning as Global Frontier Project ( CISS-2011-0031870 ), the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) ( 2014053108 ) and the BioNano Research Center funded by the Ministry of Science, ICT & Future Planning (MSIP) of Korea as Global Frontier Project ( H-GUARD_2013M3A6B2078961 ).
© 2015 Elsevier B.V.
- Bioelectronic nose
- Carbon nanotube
- Field-effect transistor
- Human olfactory receptor
- Water quality monitoring