Real-time detection of DNA cleavage induced by [M(2,2′-bipyridine) 2(NO3)](NO3) (M = Cu(II), Zn(II) and Cd(II)) complexes using linear dichroism technique

Hee Jin Park; Ji Hye Kwon; Tae Sub Cho; Jong Moon Kim; In Hong Hwang; Cheal Kim; Soojin Kim; Jinheung Kim; Seog K. Kim

doi:10.1016/j.jinorgbio.2013.06.007

Real-time detection of DNA cleavage induced by [M(2,2′-bipyridine) ₂(NO₃)](NO₃) (M = Cu(II), Zn(II) and Cd(II)) complexes using linear dichroism technique

Hee Jin Park, Ji Hye Kwon, Tae Sub Cho, Jong Moon Kim, In Hong Hwang, Cheal Kim, Soojin Kim, Jinheung Kim, Seog K. Kim

Chemistry & Nanoscience

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

The catalytic effect of [M(2,2′-bipyridine)₂(NO ₃)](NO₃) (M(bpy)₂, M = Cu(II), Zn(II) and Cd(II)) on the super-coiled and double stranded DNA (scDNA and dsDNA) was examined by electrophoresis and a real-time detection linear dichroism (LD) technique. Although the Cu(bpy)₂ complex effectively cleaved both types of DNA, the other two complexes were inactive. This was explained by the electrochemical properties of the metal complexes. The Cu(bpy)₂ complex exhibited a redox potential at - 0.222 V with a peak to peak separation of 0.201 V, whereas the other two metal complexes did not undergo any redox reaction. Both electrophoresis and LD measurements revealed the superoxide radical, ·O₂^-, to be responsible for DNA cleavage. A kinetic study using the LD technique showed that the cleavage of dsDNA consisted of two first order reactions. The fast reaction is believed to reflect the cleavage of one strand, whereas the slow reaction involves the cleavage of the complementary strand at or near the first cleaved site.

Original language	English
Pages (from-to)	46-52
Number of pages	7
Journal	Journal of Inorganic Biochemistry
Volume	127
DOIs	https://doi.org/10.1016/j.jinorgbio.2013.06.007
State	Published - 2013

Keywords

DNA cleavage
Linear dichroism
M(2,2′-bipyridine) complex
Oxidation

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10.1016/j.jinorgbio.2013.06.007

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@article{1cb1bb727abc4d439f8d9198777bb206,

title = "Real-time detection of DNA cleavage induced by [M(2,2′-bipyridine) 2(NO3)](NO3) (M = Cu(II), Zn(II) and Cd(II)) complexes using linear dichroism technique",

abstract = "The catalytic effect of [M(2,2′-bipyridine)2(NO 3)](NO3) (M(bpy)2, M = Cu(II), Zn(II) and Cd(II)) on the super-coiled and double stranded DNA (scDNA and dsDNA) was examined by electrophoresis and a real-time detection linear dichroism (LD) technique. Although the Cu(bpy)2 complex effectively cleaved both types of DNA, the other two complexes were inactive. This was explained by the electrochemical properties of the metal complexes. The Cu(bpy)2 complex exhibited a redox potential at - 0.222 V with a peak to peak separation of 0.201 V, whereas the other two metal complexes did not undergo any redox reaction. Both electrophoresis and LD measurements revealed the superoxide radical, ·O2-, to be responsible for DNA cleavage. A kinetic study using the LD technique showed that the cleavage of dsDNA consisted of two first order reactions. The fast reaction is believed to reflect the cleavage of one strand, whereas the slow reaction involves the cleavage of the complementary strand at or near the first cleaved site.",

keywords = "DNA cleavage, Linear dichroism, M(2,2′-bipyridine) complex, Oxidation",

author = "Park, {Hee Jin} and Kwon, {Ji Hye} and Cho, {Tae Sub} and Kim, {Jong Moon} and Hwang, {In Hong} and Cheal Kim and Soojin Kim and Jinheung Kim and Kim, {Seog K.}",

note = "Funding Information: This study was supported by the National Research Foundation (grant nos. 2012-008875 conferred to S.K. Kim and SRC program 2011-0001335 to J. Kim). ",

year = "2013",

doi = "10.1016/j.jinorgbio.2013.06.007",

language = "English",

volume = "127",

pages = "46--52",

journal = "Journal of Inorganic Biochemistry",

issn = "0162-0134",

publisher = "Elsevier Inc.",

}

TY - JOUR

T1 - Real-time detection of DNA cleavage induced by [M(2,2′-bipyridine) 2(NO3)](NO3) (M = Cu(II), Zn(II) and Cd(II)) complexes using linear dichroism technique

AU - Park, Hee Jin

AU - Kwon, Ji Hye

AU - Cho, Tae Sub

AU - Kim, Jong Moon

AU - Hwang, In Hong

AU - Kim, Cheal

AU - Kim, Soojin

AU - Kim, Jinheung

AU - Kim, Seog K.

N1 - Funding Information: This study was supported by the National Research Foundation (grant nos. 2012-008875 conferred to S.K. Kim and SRC program 2011-0001335 to J. Kim).

PY - 2013

Y1 - 2013

N2 - The catalytic effect of [M(2,2′-bipyridine)2(NO 3)](NO3) (M(bpy)2, M = Cu(II), Zn(II) and Cd(II)) on the super-coiled and double stranded DNA (scDNA and dsDNA) was examined by electrophoresis and a real-time detection linear dichroism (LD) technique. Although the Cu(bpy)2 complex effectively cleaved both types of DNA, the other two complexes were inactive. This was explained by the electrochemical properties of the metal complexes. The Cu(bpy)2 complex exhibited a redox potential at - 0.222 V with a peak to peak separation of 0.201 V, whereas the other two metal complexes did not undergo any redox reaction. Both electrophoresis and LD measurements revealed the superoxide radical, ·O2-, to be responsible for DNA cleavage. A kinetic study using the LD technique showed that the cleavage of dsDNA consisted of two first order reactions. The fast reaction is believed to reflect the cleavage of one strand, whereas the slow reaction involves the cleavage of the complementary strand at or near the first cleaved site.

AB - The catalytic effect of [M(2,2′-bipyridine)2(NO 3)](NO3) (M(bpy)2, M = Cu(II), Zn(II) and Cd(II)) on the super-coiled and double stranded DNA (scDNA and dsDNA) was examined by electrophoresis and a real-time detection linear dichroism (LD) technique. Although the Cu(bpy)2 complex effectively cleaved both types of DNA, the other two complexes were inactive. This was explained by the electrochemical properties of the metal complexes. The Cu(bpy)2 complex exhibited a redox potential at - 0.222 V with a peak to peak separation of 0.201 V, whereas the other two metal complexes did not undergo any redox reaction. Both electrophoresis and LD measurements revealed the superoxide radical, ·O2-, to be responsible for DNA cleavage. A kinetic study using the LD technique showed that the cleavage of dsDNA consisted of two first order reactions. The fast reaction is believed to reflect the cleavage of one strand, whereas the slow reaction involves the cleavage of the complementary strand at or near the first cleaved site.

KW - DNA cleavage

KW - Linear dichroism

KW - M(2,2′-bipyridine) complex

KW - Oxidation

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U2 - 10.1016/j.jinorgbio.2013.06.007

DO - 10.1016/j.jinorgbio.2013.06.007

M3 - Article

C2 - 23850668

AN - SCOPUS:84884410472

SN - 0162-0134

VL - 127

SP - 46

EP - 52

JO - Journal of Inorganic Biochemistry

JF - Journal of Inorganic Biochemistry