Rational Design of a Highly Selective Near-Infrared Two-Photon Fluorogenic Probe for Imaging Orthotopic Hepatocellular Carcinoma Chemotherapy

Xiaofeng Wu, Rui Wang, Sujie Qi, Nahyun Kwon, Jingjing Han, Heejeong Kim, Haidong Li, Fabiao Yu, Juyoung Yoon

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

Selective fluorescence imaging of biomarkers in vivo and in situ for evaluating orthotopic hepatocellular carcinoma (HCC) chemotherapy remains a great challenge due to current imaging agents suffering from the potential interferences of other hydrolases. Herein, we observed that carbamate unit showed a high selectivity toward the HCC-related biomarker carboxylesterase (CE) for evaluation of treatment. A near-infrared two-photon fluorescent probe was developed to not only specially image CE activity in vivo and in situ but also target orthotopic liver tumor after systemic administration. The in vivo signals of the probe correlate well with tumor apoptosis, making it possible to evaluate the status of treatment. The probe enables the imaging of CE activity in situ with a high-resolution three-dimensional view for the first time. This study may promote advances in optical imaging approaches for precise imaging-guided diagnosis of HCC in situ and its evaluation of treatment.

Original languageEnglish
Pages (from-to)15418-15425
Number of pages8
JournalAngewandte Chemie - International Edition
Volume60
Issue number28
DOIs
StatePublished - 5 Jul 2021

Keywords

  • activatable probes
  • analytical methods
  • fluorogenic imaging
  • orthotopic hepatocellular carcinoma
  • specific substrates

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