Ratiometric Two-Photon Fluorescent Probe for Detecting and Imaging Hypochlorite

Yen Leng Pak, Sang Jun Park, Qingling Xu, Hwan Myung Kim, Juyoung Yoon

Research output: Contribution to journalArticlepeer-review

88 Scopus citations

Abstract

The ratiometric fluorescent probe B6S, which contains pyrene as a fluorophore and imidazoline-2-thione as a reactive site, was developed for detection of hypochlorite (OCl-). B6S displays a high specificity toward OCl- in contrast to other reactive oxygen species and reactive nitrogen species. The probe has a low detection limit and operates under biological conditions. Moreover, the low cytotoxicity of B6S enables it to be utilized effectively for OCl- imaging in living cells and tissues by using two-photon microscopy. The findings indicate that B6S has the capability of serving as a probe to explore the biological functions of OCl- in living systems.

Original languageEnglish
Pages (from-to)9510-9514
Number of pages5
JournalAnalytical Chemistry
Volume90
Issue number15
DOIs
StatePublished - 7 Aug 2018

Bibliographical note

Funding Information:
This study was supported financially by the National Research Foundation of Korea (Grants 2012R1A3A2048814 to J.Y.; 2016R1E1A1A02920873 to H.M.K.) and the National Natural Science Foundation of China (Grant 21708041 to Q.X.). The Korea Basic Science Institute (Western Seoul) is acknowledged for the LC/MS data. FAB mass spectrometric data were performed by using a Jeol JMS 700 high resolution mass spectrometer at Korea Basic Science Institute (Daegu).

Publisher Copyright:
© 2018 American Chemical Society.

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