Rat osteopontin antibody is cross-reactive to a novel myelin-associated protein in chick

Tae Cheon Kang; Gye Sun Jeon; Hyun Joon Kim; Dong Hoon Shin; Kyung Hoon Lee; Hwa Young Lee; Young Bok Yoo; Byung Lan Lee; Sa Sun Cho

doi:10.1016/S0006-8993(98)01265-7

Rat osteopontin antibody is cross-reactive to a novel myelin-associated protein in chick

Tae Cheon Kang, Gye Sun Jeon, Hyun Joon Kim, Dong Hoon Shin, Kyung Hoon Lee, Hwa Young Lee, Young Bok Yoo, Byung Lan Lee, Sa Sun Cho

Department of Anatomy

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Osteopontin (OPN) was initially identified as glycosylated phosphoprotein in bones of vertebrates. Recently, OPN is reported to express in the primitive neuroepithelia of early chick embryonic hindbrain. We have demonstrated that rat OPN is immunohistochemically localized in the white matter of chick CNS. We have further confirmed the specificity of OPN cross- immunoreaction in myelin using demyelinated optic nerve induced by lysophosphatidylcholine (LPC), where the intensity of immunoreaction was closely related to the degree of demyelination. Immunoblot analyses showed that rat OPN antibody recognized a protein with molecular weights of ~47 kDa from chick CNS. Our data suggest that the antigen recognized by rat OPN is a previously undescribed myelin-associated protein in the chick CNS.

Original language	English
Pages (from-to)	527-530
Number of pages	4
Journal	Brain Research
Volume	818
Issue number	2
DOIs	https://doi.org/10.1016/S0006-8993(98)01265-7
State	Published - 13 Feb 1999

Keywords

Chick
Demyelination
Immunohistochemistry
Myelin
Osteopontin
Western blot

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10.1016/S0006-8993(98)01265-7

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title = "Rat osteopontin antibody is cross-reactive to a novel myelin-associated protein in chick",

abstract = "Osteopontin (OPN) was initially identified as glycosylated phosphoprotein in bones of vertebrates. Recently, OPN is reported to express in the primitive neuroepithelia of early chick embryonic hindbrain. We have demonstrated that rat OPN is immunohistochemically localized in the white matter of chick CNS. We have further confirmed the specificity of OPN cross- immunoreaction in myelin using demyelinated optic nerve induced by lysophosphatidylcholine (LPC), where the intensity of immunoreaction was closely related to the degree of demyelination. Immunoblot analyses showed that rat OPN antibody recognized a protein with molecular weights of ~47 kDa from chick CNS. Our data suggest that the antigen recognized by rat OPN is a previously undescribed myelin-associated protein in the chick CNS.",

keywords = "Chick, Demyelination, Immunohistochemistry, Myelin, Osteopontin, Western blot",

author = "Kang, {Tae Cheon} and Jeon, {Gye Sun} and Kim, {Hyun Joon} and Shin, {Dong Hoon} and Lee, {Kyung Hoon} and Lee, {Hwa Young} and Yoo, {Young Bok} and Lee, {Byung Lan} and Cho, {Sa Sun}",

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doi = "10.1016/S0006-8993(98)01265-7",

language = "English",

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pages = "527--530",

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T1 - Rat osteopontin antibody is cross-reactive to a novel myelin-associated protein in chick

AU - Kang, Tae Cheon

AU - Jeon, Gye Sun

AU - Kim, Hyun Joon

AU - Shin, Dong Hoon

AU - Lee, Kyung Hoon

AU - Lee, Hwa Young

AU - Yoo, Young Bok

AU - Lee, Byung Lan

AU - Cho, Sa Sun

PY - 1999/2/13

Y1 - 1999/2/13

N2 - Osteopontin (OPN) was initially identified as glycosylated phosphoprotein in bones of vertebrates. Recently, OPN is reported to express in the primitive neuroepithelia of early chick embryonic hindbrain. We have demonstrated that rat OPN is immunohistochemically localized in the white matter of chick CNS. We have further confirmed the specificity of OPN cross- immunoreaction in myelin using demyelinated optic nerve induced by lysophosphatidylcholine (LPC), where the intensity of immunoreaction was closely related to the degree of demyelination. Immunoblot analyses showed that rat OPN antibody recognized a protein with molecular weights of ~47 kDa from chick CNS. Our data suggest that the antigen recognized by rat OPN is a previously undescribed myelin-associated protein in the chick CNS.

AB - Osteopontin (OPN) was initially identified as glycosylated phosphoprotein in bones of vertebrates. Recently, OPN is reported to express in the primitive neuroepithelia of early chick embryonic hindbrain. We have demonstrated that rat OPN is immunohistochemically localized in the white matter of chick CNS. We have further confirmed the specificity of OPN cross- immunoreaction in myelin using demyelinated optic nerve induced by lysophosphatidylcholine (LPC), where the intensity of immunoreaction was closely related to the degree of demyelination. Immunoblot analyses showed that rat OPN antibody recognized a protein with molecular weights of ~47 kDa from chick CNS. Our data suggest that the antigen recognized by rat OPN is a previously undescribed myelin-associated protein in the chick CNS.

KW - Chick

KW - Demyelination

KW - Immunohistochemistry

KW - Myelin

KW - Osteopontin

KW - Western blot

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DO - 10.1016/S0006-8993(98)01265-7

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AN - SCOPUS:0344689928

SN - 0006-8993

VL - 818

SP - 527

EP - 530

JO - Brain Research

JF - Brain Research

IS - 2

ER -

Rat osteopontin antibody is cross-reactive to a novel myelin-associated protein in chick

Abstract

Keywords

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