TY - GEN
T1 - Rapid uropathogenic bacterial detection by integrated photonic PCR on chip with pathogenic enrichment
AU - Cho, Byungrae
AU - Lee, Jong Hwan
AU - Lee, Sang Hun
AU - Bhattacharjee, Saptati
AU - Feng, Jeffrey
AU - Hong, Soon Gweon
AU - Song, Jihwan
AU - Song, Minsun
AU - Bang, Doyeon
AU - Wang, Bowen
AU - Riley, Lee W.
AU - Lee, Luke P.
N1 - Funding Information:
The authors acknowledge the financial support provided by the National Institutes of Health (NIH) (R01 AI117064-01).
Publisher Copyright:
Copyright © (2018) by Chemical and Biological Microsystems Society. All rights reserved.
PY - 2018
Y1 - 2018
N2 - It is important to diagnose pathogenic infections rapidly in urine samples for the management and treatment. However, the conventional culture-based method is too slow (1-3 days). Here, we show an integrated PCR system on chip to identify bacteria in 15 minutes. Using a plasmonic porous membrane, we captured and concentrated 50,000-fold bacteria on the membrane from 1 ml of samples in 3 minutes without any external equipment. Due to the plasmonic photothermal effect, bacteria on the plasmonic nanoporous membrane were thermally lysed within 1 minute. Furthermore, we demonstrated photonic PCR based nucleic acid detection in 8.8 minutes for 40 cycles.
AB - It is important to diagnose pathogenic infections rapidly in urine samples for the management and treatment. However, the conventional culture-based method is too slow (1-3 days). Here, we show an integrated PCR system on chip to identify bacteria in 15 minutes. Using a plasmonic porous membrane, we captured and concentrated 50,000-fold bacteria on the membrane from 1 ml of samples in 3 minutes without any external equipment. Due to the plasmonic photothermal effect, bacteria on the plasmonic nanoporous membrane were thermally lysed within 1 minute. Furthermore, we demonstrated photonic PCR based nucleic acid detection in 8.8 minutes for 40 cycles.
UR - http://www.scopus.com/inward/record.url?scp=85079739414&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85079739414
T3 - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
SP - 1809
EP - 1811
BT - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
PB - Chemical and Biological Microsystems Society
T2 - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Y2 - 11 November 2018 through 15 November 2018
ER -