Rapid, simultaneous and nanomolar determination of pyroglutamic acid and cis-/trans-urocanic acid in human stratum corneum by hydrophilic interaction liquid chromatography (HILIC)-electrospray ionization tandem mass spectrometry

Kyung Mi Joo, Ji Yeon Han, Eui Dong Son, Gae Won Nam, Han Young Chung, Hye Jin Jeong, Jun Cheol Cho, Kyung Min Lim

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

A rapid, sensitive and specific hydrophilic interaction liquid chromatography coupled to tandem mass spectrometric (HILIC-MS/MS) method for the simultaneous determination of pyroglutamic acid, . cis- and . trans-urocanic acid in human skin stratum corneum (SC) were developed and validated. This method was carried out without derivatization or addition of ion-pair additives in mobile phase. The analytes were extracted by PBS buffer solution and analyzed using an electrospray positive ionization mass spectrometry in the multiple reaction monitoring (MRM) mode. Chromatographic separation was performed on an AQUITY UPLC amide column using gradient elution with the mobile phase of water and acetonitrile. The standard curves were linear over the concentration range of 1.0-250. ng/mL with a correlation coefficient higher than 0.999 with an LLOQ of 0.5. ng/mL. The lower limits of detection (LLOD) of these analytes were lower than 0.2. ng/mL. The intra- and inter-day precisions were measured to be below 7.7% and accuracies were within the range of 94.3-102.6%. The validated method was successfully applied to determine the level of pyroglutamic acid and . cis-/. trans-urocanic acid in the SC samples from forearm and forehead region of 19 human volunteers.

Original languageEnglish
Pages (from-to)55-63
Number of pages9
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume897
DOIs
StatePublished - 15 May 2012

Keywords

  • Cis-/trans-Urocanic acid
  • HILIC-MS/MS
  • Human stratum corneum
  • Natural moisturizing factor (NMF)
  • Pyroglutamic acid

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