Rapid separation and capture of platelets from whole blood

L. Basabe-Desmonts; S. Ramstrom; G. Meade; S. O'Neill; A. Riaz; L. Kent; D. Kenny; L. P. Lee; A. J. Ricco

Rapid separation and capture of platelets from whole blood

L. Basabe-Desmonts, S. Ramstrom, G. Meade, S. O'Neill, A. Riaz, L. Kent, D. Kenny, L. P. Lee, A. J. Ricco

Department of Chemistry & Nanoscience

Research output: Contribution to conference › Paper › peer-review

Abstract

We have designed and fabricated a physiologically inspired microfluidic device to capture and separate platelets from whole blood using selective adhesion molecules. It is comprised of a protein-patterned surface on glass, assembled with a PDMS channel. The dimensions of the protein patterns enable control of the number of platelets captured per spot; for the first time, ordered arrays of captured single or multiple platelets are easily created. A range of proteins can be patterned on the surface to control captured platelet activation and morphology, including antibodies that minimize capture-induced activation.

Original language	English
Pages	817-819
Number of pages	3
State	Published - 2008
Event	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States Duration: 12 Oct 2008 → 16 Oct 2008

Conference

Conference	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
Country/Territory	United States
City	San Diego, CA
Period	12/10/08 → 16/10/08

Keywords

Microcontact printing
PDMS microfluidics
Platelet adhesion
Protein patterning
Single platelet

Cite this

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title = "Rapid separation and capture of platelets from whole blood",

abstract = "We have designed and fabricated a physiologically inspired microfluidic device to capture and separate platelets from whole blood using selective adhesion molecules. It is comprised of a protein-patterned surface on glass, assembled with a PDMS channel. The dimensions of the protein patterns enable control of the number of platelets captured per spot; for the first time, ordered arrays of captured single or multiple platelets are easily created. A range of proteins can be patterned on the surface to control captured platelet activation and morphology, including antibodies that minimize capture-induced activation.",

keywords = "Microcontact printing, PDMS microfluidics, Platelet adhesion, Protein patterning, Single platelet",

author = "L. Basabe-Desmonts and S. Ramstrom and G. Meade and S. O'Neill and A. Riaz and L. Kent and D. Kenny and Lee, {L. P.} and Ricco, {A. J.}",

year = "2008",

language = "English",

pages = "817--819",

note = "12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 ; Conference date: 12-10-2008 Through 16-10-2008",

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TY - CONF

T1 - Rapid separation and capture of platelets from whole blood

AU - Basabe-Desmonts, L.

AU - Ramstrom, S.

AU - Meade, G.

AU - O'Neill, S.

AU - Riaz, A.

AU - Kent, L.

AU - Kenny, D.

AU - Lee, L. P.

AU - Ricco, A. J.

PY - 2008

Y1 - 2008

N2 - We have designed and fabricated a physiologically inspired microfluidic device to capture and separate platelets from whole blood using selective adhesion molecules. It is comprised of a protein-patterned surface on glass, assembled with a PDMS channel. The dimensions of the protein patterns enable control of the number of platelets captured per spot; for the first time, ordered arrays of captured single or multiple platelets are easily created. A range of proteins can be patterned on the surface to control captured platelet activation and morphology, including antibodies that minimize capture-induced activation.

AB - We have designed and fabricated a physiologically inspired microfluidic device to capture and separate platelets from whole blood using selective adhesion molecules. It is comprised of a protein-patterned surface on glass, assembled with a PDMS channel. The dimensions of the protein patterns enable control of the number of platelets captured per spot; for the first time, ordered arrays of captured single or multiple platelets are easily created. A range of proteins can be patterned on the surface to control captured platelet activation and morphology, including antibodies that minimize capture-induced activation.

KW - Microcontact printing

KW - PDMS microfluidics

KW - Platelet adhesion

KW - Protein patterning

KW - Single platelet

UR - http://www.scopus.com/inward/record.url?scp=84902492676&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:84902492676

SP - 817

EP - 819

T2 - 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008

Y2 - 12 October 2008 through 16 October 2008

ER -

Rapid separation and capture of platelets from whole blood

Abstract

Conference

Keywords

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Cite this