Raised lateral patch clamp array in open-access fluidic system

Adrian Y. Lau, Paul J. Hung, Luke P. Lee

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Scopus citations

Abstract

A novel lateral patch-clamp array with cell trapping locations raised above the substrate was developed. The fabrication method creates an open-access fluidic system by involving both microfabrication and macro-machining processes. This paper demonstrates the capability of using such an open-access fluidic system for high throughput patch clamp measurements. Seal resistances over 350MΩ were measured consistently. Patch clamp recording were obtained with from Kv2.1 potassium transfected CHO cells.

Original languageEnglish
Title of host publicationMicro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference
Subtitle of host publication9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherTransducer Research Foundation
Pages442-444
Number of pages3
ISBN (Print)0974361119, 9780974361116
StatePublished - 2005
Event9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005 - Boston, MA, United States
Duration: 9 Oct 200513 Oct 2005

Publication series

NameMicro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Volume1

Conference

Conference9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005
Country/TerritoryUnited States
CityBoston, MA
Period9/10/0513/10/05

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