Raised lateral patch clamp array

P. J. Hung, A. Y. Lau, L. P. Lee

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

A novel lateral patch-clamp array with cell trapping locations raised above the substrate was fabricated by integration of macro-machining and soft lithography technology. The device is made of PDMS (Poly-Di-Methyl-Siloxane) and is composed of four compartments: multiple lateral patch opening "raised" above the bottom of the glass slide at a pre-determined distance, an open chamber machined by a hole-puncher for fluidic access, multiple microfluidic channels to introduce cells and reagents to the patch openings, and several microfluidic channels as electrical ground for patch clamp recording. Size of the patch openings were 2 μm x 2 μm. Seal resistances over 350MO were measured consistently. The device is capable of conducting whole-cell patch-clamp experiments at lower cost and higher throughput.

Original languageEnglish
Title of host publication2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology
Pages368-370
Number of pages3
DOIs
StatePublished - 2005
Event2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology - Oahu, HI, United States
Duration: 12 May 200515 May 2005

Publication series

Name2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology
Volume2005

Conference

Conference2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology
Country/TerritoryUnited States
CityOahu, HI
Period12/05/0515/05/05

Keywords

  • Array
  • Ion channel
  • Lateral
  • Patch clamp
  • Raised

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