Raineyella fluvialis sp. Nov., an actinobacterium isolated from freshwater sediment

Yeon Bee Kim, Joon Yong Kim, Hye Seon Song, Changsu Lee, Juseok Kim, Tae Woong Whon, Se Hee Lee, Jin Kyu Rhee, Seong Woon Roh

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Abstract

A novel, facultatively anaerobic actinobacterium, designated strain CBA3103T, was isolated from sediment of the Geum River in South Korea. Phylogenetic analysis indicated that strain CBA3103T is most closely related to Raineyella antarctica LZ-22T (98.47% 16S rRNA gene sequence similarity). The genome of strain CBA3103T was 3649865 bp with a DNA G+C content of 69.6 mol%. The average nucleotide identity value between strain CBA3103T and R. antarctica LZ-22T was 79.22%. Cells of strain CBA3103T were Gram-positive, rod-shaped, 0.6–0.9 µm wide and 1.4–2.4 µm long. Growth occurred at 15–40 °C (optimum, 35 °C), at pH 6.0–7.0 (optimum, pH 7.0) and with 0–2% NaCl (w/v) (optimum, 0–1%, w/v). The major cellular fatty acids in strain CBA3103T were anteiso-C15:0, anteiso-C15:1 A and iso-C14:0. The major respiratory quinone was menaquinone-9(H4). The polar lipids of strain CBA3103T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, five unidentified glycolipids and three unidentified phospholipids. Based on the genotypic, phenotypic and chemotaxonomic analyses, strain CBA3103T represents a novel species of the genus Raineyella, for which the name Raineyella fluvialis sp. nov. (type strain CBA3103T=KACC 21446T=DSM 110288T) is proposed.

Original languageEnglish
Article number004289
Pages (from-to)4298-4304
Number of pages7
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume70
Issue number7
DOIs
StatePublished - 2020

Bibliographical note

Funding Information:
This research was supported by the ‘Survey of freshwater organisms (Prokaryotes)’ (NNIBR201901213) research programme of the Nakdonggang National Institute of Biological Resources, World Institute of Kimchi, funded by the Ministry of Science and ICT (KE2001-2), the Basic Science Research Program of the National Research Foundation of Korea (NRF) (2018R1D1A1A09082921 and 2018R1D1A1B07045349), and the Ewha Womans University Research Grant of 2019, Republic of Korea.

Publisher Copyright:
© 2020 The Authors.

Keywords

  • Actinobacteria
  • Raineyella fluvialis
  • Taxonomy

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