Raineyella fluvialis sp. Nov., an actinobacterium isolated from freshwater sediment

A novel, facultatively anaerobic actinobacterium, designated strain CBA3103^T, was isolated from sediment of the Geum River in South Korea. Phylogenetic analysis indicated that strain CBA3103^T is most closely related to Raineyella antarctica LZ-22^T (98.47% 16S rRNA gene sequence similarity). The genome of strain CBA3103^T was 3649865 bp with a DNA G+C content of 69.6 mol%. The average nucleotide identity value between strain CBA3103^T and R. antarctica LZ-22^T was 79.22%. Cells of strain CBA3103^T were Gram-positive, rod-shaped, 0.6–0.9 µm wide and 1.4–2.4 µm long. Growth occurred at 15–40 °C (optimum, 35 °C), at pH 6.0–7.0 (optimum, pH 7.0) and with 0–2% NaCl (w/v) (optimum, 0–1%, w/v). The major cellular fatty acids in strain CBA3103^T were anteiso-C_15:0, anteiso-C_15:1 A and iso-C_14:0. The major respiratory quinone was menaquinone-9(H₄). The polar lipids of strain CBA3103^T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, five unidentified glycolipids and three unidentified phospholipids. Based on the genotypic, phenotypic and chemotaxonomic analyses, strain CBA3103^T represents a novel species of the genus Raineyella, for which the name Raineyella fluvialis sp. nov. (type strain CBA3103^T=KACC 21446^T=DSM 110288^T) is proposed.