TY - JOUR
T1 - Radiation augments a sequential program of differentiation in PKC inhibitor-pretreated mouse epidermal cells
AU - Yi, Min Jeong
AU - Kim, Tae Hwan
AU - Lee, Su Jae
AU - Cho, Chul Koo
AU - Bae, In Hwa
AU - Lee, Yun Sil
PY - 1999/9
Y1 - 1999/9
N2 - The aim of this study was to determine whether γ-rays affect differentiation in mouse epidermal cells. After a pre-treatment with the PKC inhibitor staurosporin (STS) or 1-(5-isoquinolinesulfomyl)-2-methylpiperazine (H7), γ-rays were irradiated with or without an elevation of 0.12 mM Ca2+ and expressions of differentiation markers and each PKC isozyme were examined in normal primary and v-rasHa transformed mouse keratinocytes. Gamma-rays induced the expressions of differentiation markers of keratin 1 and 10 (K1 and 10), filaggrin, loricrin and SPR-1 in normal keratinocytes when the Ca2+ concentration was increased, and these phenomena were augmented in H7 pretreated cells. Similar results were obtained in STS pretreated cells; in this case, γ-rays enhanced the expressions of the differentiation markers even without an elevated Ca2+ concentration. In v-rasHa transformed cells, γ-rays induced the expression of differentiation markers not only at 0.05 mM Ca2+, but in 0.12 mM Ca2+-shifted cells, and in H7 pretreated cells, these phenomena were augmented. The translocation of PKCα to the paniculate fraction was seen in H7 pretreated normal keratinocytes. Radiation also induced PKCα expression in STS pretreated cells, independent of Ca2+-shift, as well as altered expressions of PKCδ and -η, while expressions of PKCα, -δ, -ε, and -η were enhanced in v-rasHa transformed cells. In conclusion, γ-rays augmented the expressions of both spinous and granular differentiation markers in normal and v-rasHa transformed keratinocytes and this effect was augmented when PKC inhibitors were used, which may be mediated by the cellular redistribution of PKC isozymes.
AB - The aim of this study was to determine whether γ-rays affect differentiation in mouse epidermal cells. After a pre-treatment with the PKC inhibitor staurosporin (STS) or 1-(5-isoquinolinesulfomyl)-2-methylpiperazine (H7), γ-rays were irradiated with or without an elevation of 0.12 mM Ca2+ and expressions of differentiation markers and each PKC isozyme were examined in normal primary and v-rasHa transformed mouse keratinocytes. Gamma-rays induced the expressions of differentiation markers of keratin 1 and 10 (K1 and 10), filaggrin, loricrin and SPR-1 in normal keratinocytes when the Ca2+ concentration was increased, and these phenomena were augmented in H7 pretreated cells. Similar results were obtained in STS pretreated cells; in this case, γ-rays enhanced the expressions of the differentiation markers even without an elevated Ca2+ concentration. In v-rasHa transformed cells, γ-rays induced the expression of differentiation markers not only at 0.05 mM Ca2+, but in 0.12 mM Ca2+-shifted cells, and in H7 pretreated cells, these phenomena were augmented. The translocation of PKCα to the paniculate fraction was seen in H7 pretreated normal keratinocytes. Radiation also induced PKCα expression in STS pretreated cells, independent of Ca2+-shift, as well as altered expressions of PKCδ and -η, while expressions of PKCα, -δ, -ε, and -η were enhanced in v-rasHa transformed cells. In conclusion, γ-rays augmented the expressions of both spinous and granular differentiation markers in normal and v-rasHa transformed keratinocytes and this effect was augmented when PKC inhibitors were used, which may be mediated by the cellular redistribution of PKC isozymes.
KW - Differentiation markers
KW - Gamma-ray
KW - Mouse epidermal cell
KW - PKC
KW - v-ras
UR - http://www.scopus.com/inward/record.url?scp=0033182850&partnerID=8YFLogxK
U2 - 10.1269/jrr.40.273
DO - 10.1269/jrr.40.273
M3 - Article
C2 - 10641489
AN - SCOPUS:0033182850
SN - 0449-3060
VL - 40
SP - 273
EP - 284
JO - Journal of Radiation Research
JF - Journal of Radiation Research
IS - 3
ER -