Quantitative determination of daumone in rat plasma by liquid chromatography-mass spectrometry

Keumhan Noh, Jung Hyun Park, Jong Hee Park, Minkyu Kim, Mankil Jung, Hunjoo Ha, Kwang il Kwon, Hwa Jeong Lee, Wonku Kang

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


Daumone, 6-(3,5-dihydroxy-6-methyl-tetrahydro-pyran-2-yloxy)-heptanoic acid is a pheromone secreted by Caenorhabditis elegans, and has been known as a pivotal regulator of chemosensory processes in development and ageing. A quantification method using mass spectrometry was developed for the determination of daumone in rat plasma. After simple protein precipitation with acetonitrile including an internal standard, the analytes were chromatographed on a reversed-phase column and detected by liquid chromatography/tandem mass spectrometry with electrospray ionization. The accuracy and precision of the assay were in accordance with FDA regulations for validation of bioanalytical methods. This method was applied to measure the plasma daumone concentrations after a single intravenous administration of daumone in rats.

Original languageEnglish
Pages (from-to)114-117
Number of pages4
JournalJournal of Pharmaceutical and Biomedical Analysis
Issue number1
StatePublished - 25 Aug 2011

Bibliographical note

Funding Information:
This research was supported by the Yeungnam University Research Grants in 2010 ( 210-A-054-025 ).


  • Daumone
  • LC-MS/MS
  • Rat plasma


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