Quantitative determination of daumone in rat plasma by liquid chromatography-mass spectrometry

Keumhan Noh; Jung Hyun Park; Jong Hee Park; Minkyu Kim; Mankil Jung; Hunjoo Ha; Kwang il Kwon; Hwa Jeong Lee; Wonku Kang

doi:10.1016/j.jpba.2011.04.008

Quantitative determination of daumone in rat plasma by liquid chromatography-mass spectrometry

Keumhan Noh, Jung Hyun Park, Jong Hee Park, Minkyu Kim, Mankil Jung, Hunjoo Ha, Kwang il Kwon, Hwa Jeong Lee, Wonku Kang

Department of Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Daumone, 6-(3,5-dihydroxy-6-methyl-tetrahydro-pyran-2-yloxy)-heptanoic acid is a pheromone secreted by Caenorhabditis elegans, and has been known as a pivotal regulator of chemosensory processes in development and ageing. A quantification method using mass spectrometry was developed for the determination of daumone in rat plasma. After simple protein precipitation with acetonitrile including an internal standard, the analytes were chromatographed on a reversed-phase column and detected by liquid chromatography/tandem mass spectrometry with electrospray ionization. The accuracy and precision of the assay were in accordance with FDA regulations for validation of bioanalytical methods. This method was applied to measure the plasma daumone concentrations after a single intravenous administration of daumone in rats.

Original language	English
Pages (from-to)	114-117
Number of pages	4
Journal	Journal of Pharmaceutical and Biomedical Analysis
Volume	56
Issue number	1
DOIs	https://doi.org/10.1016/j.jpba.2011.04.008
State	Published - 25 Aug 2011

Bibliographical note

Funding Information:
This research was supported by the Yeungnam University Research Grants in 2010 ( 210-A-054-025 ).

Keywords

Daumone
LC-MS/MS
Rat plasma

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10.1016/j.jpba.2011.04.008

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title = "Quantitative determination of daumone in rat plasma by liquid chromatography-mass spectrometry",

abstract = "Daumone, 6-(3,5-dihydroxy-6-methyl-tetrahydro-pyran-2-yloxy)-heptanoic acid is a pheromone secreted by Caenorhabditis elegans, and has been known as a pivotal regulator of chemosensory processes in development and ageing. A quantification method using mass spectrometry was developed for the determination of daumone in rat plasma. After simple protein precipitation with acetonitrile including an internal standard, the analytes were chromatographed on a reversed-phase column and detected by liquid chromatography/tandem mass spectrometry with electrospray ionization. The accuracy and precision of the assay were in accordance with FDA regulations for validation of bioanalytical methods. This method was applied to measure the plasma daumone concentrations after a single intravenous administration of daumone in rats.",

keywords = "Daumone, LC-MS/MS, Rat plasma",

author = "Keumhan Noh and Park, {Jung Hyun} and Park, {Jong Hee} and Minkyu Kim and Mankil Jung and Hunjoo Ha and Kwon, {Kwang il} and Lee, {Hwa Jeong} and Wonku Kang",

note = "Funding Information: This research was supported by the Yeungnam University Research Grants in 2010 ( 210-A-054-025 ).",

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doi = "10.1016/j.jpba.2011.04.008",

language = "English",

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journal = "Journal of Pharmaceutical and Biomedical Analysis",

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AU - Noh, Keumhan

AU - Park, Jung Hyun

AU - Park, Jong Hee

AU - Kim, Minkyu

AU - Jung, Mankil

AU - Ha, Hunjoo

AU - Kwon, Kwang il

AU - Lee, Hwa Jeong

AU - Kang, Wonku

N1 - Funding Information: This research was supported by the Yeungnam University Research Grants in 2010 ( 210-A-054-025 ).

PY - 2011/8/25

Y1 - 2011/8/25

N2 - Daumone, 6-(3,5-dihydroxy-6-methyl-tetrahydro-pyran-2-yloxy)-heptanoic acid is a pheromone secreted by Caenorhabditis elegans, and has been known as a pivotal regulator of chemosensory processes in development and ageing. A quantification method using mass spectrometry was developed for the determination of daumone in rat plasma. After simple protein precipitation with acetonitrile including an internal standard, the analytes were chromatographed on a reversed-phase column and detected by liquid chromatography/tandem mass spectrometry with electrospray ionization. The accuracy and precision of the assay were in accordance with FDA regulations for validation of bioanalytical methods. This method was applied to measure the plasma daumone concentrations after a single intravenous administration of daumone in rats.

AB - Daumone, 6-(3,5-dihydroxy-6-methyl-tetrahydro-pyran-2-yloxy)-heptanoic acid is a pheromone secreted by Caenorhabditis elegans, and has been known as a pivotal regulator of chemosensory processes in development and ageing. A quantification method using mass spectrometry was developed for the determination of daumone in rat plasma. After simple protein precipitation with acetonitrile including an internal standard, the analytes were chromatographed on a reversed-phase column and detected by liquid chromatography/tandem mass spectrometry with electrospray ionization. The accuracy and precision of the assay were in accordance with FDA regulations for validation of bioanalytical methods. This method was applied to measure the plasma daumone concentrations after a single intravenous administration of daumone in rats.

KW - Daumone

KW - LC-MS/MS

KW - Rat plasma

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SN - 0731-7085

VL - 56

SP - 114

EP - 117

JO - Journal of Pharmaceutical and Biomedical Analysis

JF - Journal of Pharmaceutical and Biomedical Analysis

IS - 1

ER -

Quantitative determination of daumone in rat plasma by liquid chromatography-mass spectrometry

Abstract

Bibliographical note

Keywords

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