Quantitative and qualitative analysis of autophagy flux using imaging

Suree Kim, Soohee Choi, Dongmin Kang

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

As an intracellular degradation system, autophagy is an essential and defensive cellular program required for cell survival and cellular metabolic homeostasis in response to various stresses, such as nutrient deprivation and the accumulation of damaged organelles. In general, autophagy flux consists of four steps: (1) initiation (formation of phagophore), (2) maturation and completion of autophagosome, (3) fusion of autophagosomes with lysosomes (formation of autolysosome), and (4) degradation of intravesicular components within autolysosomes. The number of genes and reagents that modulate autophagy is increasing. Investigation of their effect on autophagy flux is critical to understanding the roles of autophagy in many physiological and pathological processes. In this review, we summarize and discuss ways to analyze autophagy flux quantitatively and qualitatively with the use of imaging tools. The suggested imaging method can help estimate whether each modulator is an inhibitor or a promoter of autophagy and elucidate the mode of action of specific genes and reagents on autophagy processes.

Original languageEnglish
Pages (from-to)241-247
Number of pages7
JournalBMB Reports
Volume53
Issue number5
DOIs
StatePublished - 2020

Bibliographical note

Funding Information:
This study was supported by grants from the National Research Foundation of Korea (2019R1A6C1010020 and 2018R1D1A1A02049371) and from the Ministry of Food and Drug Safety in 2016 (16173MFDS009) in Korea.

Publisher Copyright:
© 2020 by the The Korean Society for Biochemistry and Molecular Biology.

Keywords

  • Autolysosome
  • Autophagosome
  • Autophagy
  • Fluorescence imaging
  • Quantitative analysis

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