This paper presents a clinical evaluation of the KAIST-Ewha Colonoscopy Simulator II, which includes quantitative analysis of colonoscopy experts' and trainees ' profiles during simulation. This version of the colonoscopy simulator employs an actual colonoscope with added electronics for simulation, and succeeds the previous version of the colonoscopy simulator with the specialized haptic device and proprietary colon modeler. Five colonoscopy experts participated in the profiling of the experts' colonoscopy motions. Nine subjects consisting of three fellows and six residents participated in the training efficacy evaluation of the developed simulator. Two colon models constructed from patients' CT data are used for the training and the trainees ' performance parameters are logged in real-time during simulation. Comparison of the trainees 'profiles during the first 10 trials and the last 10 trials shows that the targeted colonoscopy skills are acquired well through the simulation-based training.