Quantitative analysis of betaine in Lycii Fructus by HILIC-ELSD

Bing Tian Zhao; Su Yang Jeong; Kyoung Hwangbo; Dong Cheul Moon; Eun Kyoung Seo; Dongho Lee; Je Hyun Lee; Byung Sun Min; Eun Sook Ma; Jong Keun Son; Mi Hee Woo

doi:10.1007/s12272-013-0148-9

Quantitative analysis of betaine in Lycii Fructus by HILIC-ELSD

Bing Tian Zhao, Su Yang Jeong, Kyoung Hwangbo, Dong Cheul Moon, Eun Kyoung Seo, Dongho Lee, Je Hyun Lee, Byung Sun Min, Eun Sook Ma, Jong Keun Son, Mi Hee Woo

Department of Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

A rapid and simple high-performance liquid chromatography (HPLC) method with evaporative light scattering detection (ELSD) was developed for the determination of betaine from Lycii Fructus. Betaine was separated with an Atlantis hydrophilic interaction liquid chromatography silica column (4.6 × 150 mm, 5 μm, 100 Å) by isocratic elution using 30 mM ammonium acetate buffer and acetonitrile (20:80, v/v %) as the mobile phase. The flow rate was 1.0 mL/min, and the temperature for the spray chamber and drift tube was set at 30 and 50 C, respectively. The method was fully validated with respect to linearity, precision, accuracy, stability and robustness. The HPLC/ELSD method was applied successfully to the quantification of betaine in the extract of Lycii Fructus. The HPLC analytical method for pattern recognition analysis was validated by repeated analysis of twenty-six L. barbarum L. from China (BC01-BC26), 3 L. barbarum L. (BJ27-BJ29) from Japan, 12 L. chinense Miller from China (CC30-CC41) and 51 L. chinense Miller samples (CK42-CK92) from Korea. The results indicate that the established HPLC/ELSD method is suitable for quality evaluation of Lycii Fructus.

Original language	English
Pages (from-to)	1231-1237
Number of pages	7
Journal	Archives of Pharmacal Research
Volume	36
Issue number	10
DOIs	https://doi.org/10.1007/s12272-013-0148-9
State	Published - Oct 2013

Bibliographical note

Funding Information:
Acknowledgments This research was supported by a Grant (09112KFDA817) from the National Center for Standardization of Herbal Medicine funded by the Korea Food & Drug Administration, Republic of Korea.

Keywords

Betaine
ELSD
HPLC
Lycii Fructus
Pattern recognition
Quality control

Access to Document

10.1007/s12272-013-0148-9

Cite this

@article{7b27468f902a4c7a8f5b70677b7c3bfd,

title = "Quantitative analysis of betaine in Lycii Fructus by HILIC-ELSD",

abstract = "A rapid and simple high-performance liquid chromatography (HPLC) method with evaporative light scattering detection (ELSD) was developed for the determination of betaine from Lycii Fructus. Betaine was separated with an Atlantis hydrophilic interaction liquid chromatography silica column (4.6 × 150 mm, 5 μm, 100 {\AA}) by isocratic elution using 30 mM ammonium acetate buffer and acetonitrile (20:80, v/v %) as the mobile phase. The flow rate was 1.0 mL/min, and the temperature for the spray chamber and drift tube was set at 30 and 50 C, respectively. The method was fully validated with respect to linearity, precision, accuracy, stability and robustness. The HPLC/ELSD method was applied successfully to the quantification of betaine in the extract of Lycii Fructus. The HPLC analytical method for pattern recognition analysis was validated by repeated analysis of twenty-six L. barbarum L. from China (BC01-BC26), 3 L. barbarum L. (BJ27-BJ29) from Japan, 12 L. chinense Miller from China (CC30-CC41) and 51 L. chinense Miller samples (CK42-CK92) from Korea. The results indicate that the established HPLC/ELSD method is suitable for quality evaluation of Lycii Fructus.",

keywords = "Betaine, ELSD, HPLC, Lycii Fructus, Pattern recognition, Quality control",

author = "Zhao, {Bing Tian} and Jeong, {Su Yang} and Kyoung Hwangbo and Moon, {Dong Cheul} and Seo, {Eun Kyoung} and Dongho Lee and Lee, {Je Hyun} and Min, {Byung Sun} and Ma, {Eun Sook} and Son, {Jong Keun} and Woo, {Mi Hee}",

note = "Funding Information: Acknowledgments This research was supported by a Grant (09112KFDA817) from the National Center for Standardization of Herbal Medicine funded by the Korea Food & Drug Administration, Republic of Korea.",

year = "2013",

month = oct,

doi = "10.1007/s12272-013-0148-9",

language = "English",

volume = "36",

pages = "1231--1237",

journal = "Archives of Pharmacal Research",

issn = "0253-6269",

publisher = "Pharmaceutical Society of Korea",

number = "10",

}

TY - JOUR

T1 - Quantitative analysis of betaine in Lycii Fructus by HILIC-ELSD

AU - Zhao, Bing Tian

AU - Jeong, Su Yang

AU - Hwangbo, Kyoung

AU - Moon, Dong Cheul

AU - Seo, Eun Kyoung

AU - Lee, Dongho

AU - Lee, Je Hyun

AU - Min, Byung Sun

AU - Ma, Eun Sook

AU - Son, Jong Keun

AU - Woo, Mi Hee

N1 - Funding Information: Acknowledgments This research was supported by a Grant (09112KFDA817) from the National Center for Standardization of Herbal Medicine funded by the Korea Food & Drug Administration, Republic of Korea.

PY - 2013/10

Y1 - 2013/10

N2 - A rapid and simple high-performance liquid chromatography (HPLC) method with evaporative light scattering detection (ELSD) was developed for the determination of betaine from Lycii Fructus. Betaine was separated with an Atlantis hydrophilic interaction liquid chromatography silica column (4.6 × 150 mm, 5 μm, 100 Å) by isocratic elution using 30 mM ammonium acetate buffer and acetonitrile (20:80, v/v %) as the mobile phase. The flow rate was 1.0 mL/min, and the temperature for the spray chamber and drift tube was set at 30 and 50 C, respectively. The method was fully validated with respect to linearity, precision, accuracy, stability and robustness. The HPLC/ELSD method was applied successfully to the quantification of betaine in the extract of Lycii Fructus. The HPLC analytical method for pattern recognition analysis was validated by repeated analysis of twenty-six L. barbarum L. from China (BC01-BC26), 3 L. barbarum L. (BJ27-BJ29) from Japan, 12 L. chinense Miller from China (CC30-CC41) and 51 L. chinense Miller samples (CK42-CK92) from Korea. The results indicate that the established HPLC/ELSD method is suitable for quality evaluation of Lycii Fructus.

AB - A rapid and simple high-performance liquid chromatography (HPLC) method with evaporative light scattering detection (ELSD) was developed for the determination of betaine from Lycii Fructus. Betaine was separated with an Atlantis hydrophilic interaction liquid chromatography silica column (4.6 × 150 mm, 5 μm, 100 Å) by isocratic elution using 30 mM ammonium acetate buffer and acetonitrile (20:80, v/v %) as the mobile phase. The flow rate was 1.0 mL/min, and the temperature for the spray chamber and drift tube was set at 30 and 50 C, respectively. The method was fully validated with respect to linearity, precision, accuracy, stability and robustness. The HPLC/ELSD method was applied successfully to the quantification of betaine in the extract of Lycii Fructus. The HPLC analytical method for pattern recognition analysis was validated by repeated analysis of twenty-six L. barbarum L. from China (BC01-BC26), 3 L. barbarum L. (BJ27-BJ29) from Japan, 12 L. chinense Miller from China (CC30-CC41) and 51 L. chinense Miller samples (CK42-CK92) from Korea. The results indicate that the established HPLC/ELSD method is suitable for quality evaluation of Lycii Fructus.

KW - Betaine

KW - ELSD

KW - HPLC

KW - Lycii Fructus

KW - Pattern recognition

KW - Quality control

UR - http://www.scopus.com/inward/record.url?scp=84886921866&partnerID=8YFLogxK

U2 - 10.1007/s12272-013-0148-9

DO - 10.1007/s12272-013-0148-9

M3 - Article

C2 - 23666821

AN - SCOPUS:84886921866

SN - 0253-6269

VL - 36

SP - 1231

EP - 1237

JO - Archives of Pharmacal Research

JF - Archives of Pharmacal Research

IS - 10

ER -

Quantitative analysis of betaine in Lycii Fructus by HILIC-ELSD

Abstract

Bibliographical note

Keywords

Access to Document

Fingerprint

Cite this