@article{17b9523c8c0f42f1bed3f45b0fe08476,
title = "QSER1 protects DNA methylation valleys from de novo methylation",
abstract = "DNA methylation is essential to mammalian development, and dysregulation can cause serious pathological conditions. Key enzymes responsible for deposition and removal of DNA methylation are known, but how they cooperate to regulate the methylation landscape remains a central question. Using a knockin DNA methylation reporter, we performed a genome-wide CRISPR-Cas9 screen in human embryonic stem cells to discover DNA methylation regulators. The top screen hit was an uncharacterized gene, QSER1, which proved to be a key guardian of bivalent promoters and poised enhancers of developmental genes, especially those residing in DNA methylation valleys (or canyons). We further demonstrate genetic and biochemical interactions of QSER1 and TET1, supporting their cooperation to safeguard transcriptional and developmental programs from DNMT3-mediated de novo methylation.",
author = "Gary Dixon and Heng Pan and Dapeng Yang and Rosen, {Bess P.} and Therande Jashari and Nipun Verma and Julian Pulecio and Inbal Caspi and Kihyun Lee and Stephanie Stransky and Abigail Glezer and Chang Liu and Marco Rivas and Ritu Kumar and Yahui Lan and Ingrid Torregroza and Chuan He and Simone Sidoli and Todd Evans and Olivier Elemento and Danwei Huangfu",
note = "Funding Information: This study was funded in part by the Starr Tri-I Stem Cell Initiative (grant no. 2016-032), the NIH/NIDDK (grant no. R01DK096239), a NIH/NCI MSKCC Cancer Center Support Grant (grant no. P30CA008748), the American Diabetes Association (grant no. 1-19-IBS-125), NIH T32 Training Grants (grant no. T32HD060600 to G.D. and T32GM008539 to B.P.R.), a Frank Lappin Horsfall, Jr. Fellowship (to G.D.), a Howard Hughes Medical Institute Medical Research Fellowship (to N.V.), a postdoctoral fellowship from a NYSTEM grant (no. DOH01-TRAIN3-2015-2016-00006 to D.Y.), and the NIH/NHGRI (R01 HG006827 to C.H.). C.H. is a Howard Hughes Medical Institute investigator. Publisher Copyright: Copyright {\textcopyright} 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works",
year = "2021",
month = apr,
day = "9",
doi = "10.1126/science.abd0875",
language = "English",
volume = "372",
journal = "Science",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "6538",
}