Pyrene based fluorescent probes for detecting endogenous zinc ions in live cells

Ying Hu; Yifan Liu; Gyoungmi Kim; Eun Jin Jun; K. M.K. Swamy; Youngmee Kim; Sung Jin Kim; Juyoung Yoon

doi:10.1016/j.dyepig.2014.09.010

Pyrene based fluorescent probes for detecting endogenous zinc ions in live cells

Ying Hu, Yifan Liu, Gyoungmi Kim, Eun Jin Jun, K. M.K. Swamy, Youngmee Kim, Sung Jin Kim, Juyoung Yoon

Department of Chemistry & Nanoscience

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

Two unique pyrene derivatives are reported as Zn²⁺selective fluorescent probes, in which two pyrenes bearing imine and OH groups are connected via an ether linker or thioether linker. Upon the addition of Zn²⁺, the resulting phenolate group can induce internal charge transfer (ICT) peaks, which can cause selective turn-on fluorescence of each probe at 550 nm. Bathochromic shifts of their emissions are observed upon the binding with Zn²⁺. Each probe can successfully image exogenous Zn²⁺ions as well as free zinc ions released during apoptosis. These results using live cells suggest that these pyrene derived probes can be very effective probes in zinc biology studies.

Original language	English
Pages (from-to)	372-377
Number of pages	6
Journal	Dyes and Pigments
Volume	113
DOIs	https://doi.org/10.1016/j.dyepig.2014.09.010
State	Published - Feb 2015

Bibliographical note

Funding Information:
This research was supported by a grant from the National Creative Research Initiative programs of the National Research Foundation of Korea (NRF) funded by the Korean government (MSIP) (No. 2012R1A3A2048814 ).

Publisher Copyright:
© 2014 Elsevier Ltd. All rights reserved.

Keywords

Apoptosis
Cell imaging
Fluorescence chemosensor
Pancreatic-cells
Pyrene derivatives
Zinc ion probe

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10.1016/j.dyepig.2014.09.010

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title = "Pyrene based fluorescent probes for detecting endogenous zinc ions in live cells",

abstract = "Two unique pyrene derivatives are reported as Zn2+selective fluorescent probes, in which two pyrenes bearing imine and OH groups are connected via an ether linker or thioether linker. Upon the addition of Zn2+, the resulting phenolate group can induce internal charge transfer (ICT) peaks, which can cause selective turn-on fluorescence of each probe at 550 nm. Bathochromic shifts of their emissions are observed upon the binding with Zn2+. Each probe can successfully image exogenous Zn2+ions as well as free zinc ions released during apoptosis. These results using live cells suggest that these pyrene derived probes can be very effective probes in zinc biology studies.",

keywords = "Apoptosis, Cell imaging, Fluorescence chemosensor, Pancreatic-cells, Pyrene derivatives, Zinc ion probe",

author = "Ying Hu and Yifan Liu and Gyoungmi Kim and Jun, \{Eun Jin\} and Swamy, \{K. M.K.\} and Youngmee Kim and Kim, \{Sung Jin\} and Juyoung Yoon",

note = "Funding Information: This research was supported by a grant from the National Creative Research Initiative programs of the National Research Foundation of Korea (NRF) funded by the Korean government (MSIP) (No. 2012R1A3A2048814 ). Publisher Copyright: {\textcopyright} 2014 Elsevier Ltd. All rights reserved.",

year = "2015",

month = feb,

doi = "10.1016/j.dyepig.2014.09.010",

language = "English",

volume = "113",

pages = "372--377",

journal = "Dyes and Pigments",

issn = "0143-7208",

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T1 - Pyrene based fluorescent probes for detecting endogenous zinc ions in live cells

AU - Hu, Ying

AU - Liu, Yifan

AU - Kim, Gyoungmi

AU - Jun, Eun Jin

AU - Swamy, K. M.K.

AU - Kim, Youngmee

AU - Kim, Sung Jin

AU - Yoon, Juyoung

N1 - Funding Information: This research was supported by a grant from the National Creative Research Initiative programs of the National Research Foundation of Korea (NRF) funded by the Korean government (MSIP) (No. 2012R1A3A2048814 ). Publisher Copyright: © 2014 Elsevier Ltd. All rights reserved.

PY - 2015/2

Y1 - 2015/2

N2 - Two unique pyrene derivatives are reported as Zn2+selective fluorescent probes, in which two pyrenes bearing imine and OH groups are connected via an ether linker or thioether linker. Upon the addition of Zn2+, the resulting phenolate group can induce internal charge transfer (ICT) peaks, which can cause selective turn-on fluorescence of each probe at 550 nm. Bathochromic shifts of their emissions are observed upon the binding with Zn2+. Each probe can successfully image exogenous Zn2+ions as well as free zinc ions released during apoptosis. These results using live cells suggest that these pyrene derived probes can be very effective probes in zinc biology studies.

AB - Two unique pyrene derivatives are reported as Zn2+selective fluorescent probes, in which two pyrenes bearing imine and OH groups are connected via an ether linker or thioether linker. Upon the addition of Zn2+, the resulting phenolate group can induce internal charge transfer (ICT) peaks, which can cause selective turn-on fluorescence of each probe at 550 nm. Bathochromic shifts of their emissions are observed upon the binding with Zn2+. Each probe can successfully image exogenous Zn2+ions as well as free zinc ions released during apoptosis. These results using live cells suggest that these pyrene derived probes can be very effective probes in zinc biology studies.

KW - Apoptosis

KW - Cell imaging

KW - Fluorescence chemosensor

KW - Pancreatic-cells

KW - Pyrene derivatives

KW - Zinc ion probe

UR - https://www.scopus.com/pages/publications/84907207436

U2 - 10.1016/j.dyepig.2014.09.010

DO - 10.1016/j.dyepig.2014.09.010

M3 - Article

AN - SCOPUS:84907207436

SN - 0143-7208

VL - 113

SP - 372

EP - 377

JO - Dyes and Pigments

JF - Dyes and Pigments

ER -

Pyrene based fluorescent probes for detecting endogenous zinc ions in live cells

Abstract

Bibliographical note

Keywords

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