Proteomic identification of radiation response markers in mouse intestine and brain

Young Bin Lim, Bo Jeong Pyun, Hae June Lee, Sang Rok Jeon, Yeung Bae Jin, Yun Sil Lee

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Increasing efforts are being made to develop more sensitive and faster molecular methodologies at the genomic and proteomic levels for the identification of protein markers after exposure to ionizing radiation (IR). However, few specific protein markers, especially organ-specific markers, have been identified. In this study, we analyzed altered protein expressions in various tissues, namely, brain, lung, spleen, and intestine, from 1Gy-irradiated mice by employing 2-DE analysis. MALDI-TOF MS and peptide mapping identified 25 proteins that showed greater than twofold expressional changes. In order to confirm significant differences between control and IR-treated samples, ten identified proteins with available commercial antibodies were selected for immunoblotting. Of these, only five showed protein expression patterns that were similar to 2-DE data. These were heat shock protein 5 (HSP 5), HSP 90kDa β, HSP 1, transaldolase 1 (TA1), and phosphoglycerate kinase 1 (PGK1). In particular, PGK1 was specifically upregulated in mouse intestine, and TA1 was specifically downregulated in brain by irradiation. TA1 expression was unaltered in other tissues. Based on these data, we suggest that TA1 and PGK1 can be considered as candidate tissue-specific protein markers of IR exposure.

Original languageEnglish
Pages (from-to)1254-1263
Number of pages10
JournalProteomics
Volume11
Issue number7
DOIs
StatePublished - Apr 2011

Keywords

  • 2-DE analysis
  • Animal proteomics
  • Phosphoglycerate kinase 1
  • Radiation protein markers
  • Transaldolase 1

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