TY - JOUR
T1 - Proteomic identification of radiation response markers in mouse intestine and brain
AU - Lim, Young Bin
AU - Pyun, Bo Jeong
AU - Lee, Hae June
AU - Jeon, Sang Rok
AU - Jin, Yeung Bae
AU - Lee, Yun Sil
PY - 2011/4
Y1 - 2011/4
N2 - Increasing efforts are being made to develop more sensitive and faster molecular methodologies at the genomic and proteomic levels for the identification of protein markers after exposure to ionizing radiation (IR). However, few specific protein markers, especially organ-specific markers, have been identified. In this study, we analyzed altered protein expressions in various tissues, namely, brain, lung, spleen, and intestine, from 1Gy-irradiated mice by employing 2-DE analysis. MALDI-TOF MS and peptide mapping identified 25 proteins that showed greater than twofold expressional changes. In order to confirm significant differences between control and IR-treated samples, ten identified proteins with available commercial antibodies were selected for immunoblotting. Of these, only five showed protein expression patterns that were similar to 2-DE data. These were heat shock protein 5 (HSP 5), HSP 90kDa β, HSP 1, transaldolase 1 (TA1), and phosphoglycerate kinase 1 (PGK1). In particular, PGK1 was specifically upregulated in mouse intestine, and TA1 was specifically downregulated in brain by irradiation. TA1 expression was unaltered in other tissues. Based on these data, we suggest that TA1 and PGK1 can be considered as candidate tissue-specific protein markers of IR exposure.
AB - Increasing efforts are being made to develop more sensitive and faster molecular methodologies at the genomic and proteomic levels for the identification of protein markers after exposure to ionizing radiation (IR). However, few specific protein markers, especially organ-specific markers, have been identified. In this study, we analyzed altered protein expressions in various tissues, namely, brain, lung, spleen, and intestine, from 1Gy-irradiated mice by employing 2-DE analysis. MALDI-TOF MS and peptide mapping identified 25 proteins that showed greater than twofold expressional changes. In order to confirm significant differences between control and IR-treated samples, ten identified proteins with available commercial antibodies were selected for immunoblotting. Of these, only five showed protein expression patterns that were similar to 2-DE data. These were heat shock protein 5 (HSP 5), HSP 90kDa β, HSP 1, transaldolase 1 (TA1), and phosphoglycerate kinase 1 (PGK1). In particular, PGK1 was specifically upregulated in mouse intestine, and TA1 was specifically downregulated in brain by irradiation. TA1 expression was unaltered in other tissues. Based on these data, we suggest that TA1 and PGK1 can be considered as candidate tissue-specific protein markers of IR exposure.
KW - 2-DE analysis
KW - Animal proteomics
KW - Phosphoglycerate kinase 1
KW - Radiation protein markers
KW - Transaldolase 1
UR - http://www.scopus.com/inward/record.url?scp=79953062345&partnerID=8YFLogxK
U2 - 10.1002/pmic.201000332
DO - 10.1002/pmic.201000332
M3 - Article
C2 - 21319302
AN - SCOPUS:79953062345
SN - 1615-9853
VL - 11
SP - 1254
EP - 1263
JO - Proteomics
JF - Proteomics
IS - 7
ER -