Proteomic analysis of liver tissue from HBx-transgenic mice at early stages of hepatocarcinogenesis

Sun Young Kim, Phil Young Lee, Hye Jun Shin, Do Hyung Kim, Sunghyun Kang, Hyung Bae Moon, Sang Won Kang, Jin Man Kim, Sung Goo Park, Byoung Chul Park, Dae Yeul Yu, Kwang Hee Bae, Sang Chul Lee

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34 Scopus citations


The hepatitis B virus X-protein (HBx), a multifunctional viral regulator, participates in the viral life cycle and in the development of hepatocellular carcinoma (HCC). We previously reported a high incidence of HCC in transgenic mice expressing HBx. In this study, proteomic analysis was performed to identify proteins that may be involved in hepatocarcinogenesis and/or that could be utilized as early detection biomarkers for HCC. Proteins from the liver tissue of HBx-transgenic mice at early stages of carcinogenesis (dysplasia and hepatocellular adenoma) were separated by 2-DE, and quantitative changes were analyzed. A total of 22 spots displaying significant quantitative changes were identified using LC-MS/MS. In particular, several proteins involved in glucose and fatty acid metabolism, such as mitochondrial 3-ketoacyl-CoA thiolase, intestinal fatty acid-binding protein 2 and cytoplasmic malate dehydrogenase, were differentially expressed, implying that significant metabolic alterations occurred during the early stages of hepatocarcinogenesis. The results of this proteomic analysis provide insights into the mechanism of HBx-mediated hepatocarcinogenesis. Additionally, this study identifies possible therapeutic targets for HCC diagnosis and novel drug development for treatment of the disease.

Original languageEnglish
Pages (from-to)5056-5066
Number of pages11
Issue number22
StatePublished - Nov 2009


  • Animal proteomics
  • Dysplasia
  • Hepatitis B virus X-protein
  • Hepatocellular adenoma
  • Hepatocellular carcinoma


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