Proteomic analysis of fetal programming-related obesity markers

Ji Hye Lee, Jae Young Yoo, Young Ah You, Woo Sung Kwon, Sang Mi Lee, Myung Geol Pang, Young Ju Kim

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

The objectives of this study were to analyze fetal programming in rat brain using proteomic analysis and to identify fetal programming-related obesity markers. Sprague-Dawley rats were divided into four feeding groups: (i) the Ad Libitum (AdLib)/AdLib group was given a normal diet during pregnancy and the lactation period; (ii) the AdLib/maternal food restriction group (FR) was subjected to 50% FR during the lactation period; (iii) the FR/AdLib group was subjected to 50% FR during pregnancy; and (iv) the FR/FR group was subjected to 50% FR during pregnancy and the lactation period. Offspring from each group were sacrificed at 3 weeks of age and whole brains were dissected. To obtain a maximum number of protein markers related to obesity, 2DE and Pathway Studio bioinformatics analysis were performed. The identities of the markers among the selected and candidate proteins were confirmed by Western blotting and immunohistochemistry. Proteomic and bioinformatics analyses revealed that expression of ubiquitin carboxy-terminal hydrolase L1 (UCHL1) and Secernin 1 (SCRN1) were significantly different in the FR/AdLib group compared with the AdLib/AdLib group for both male and female offspring. These findings suggest that UCHL1 and SCRN1 may be used as fetal programming-related obesity markers.

Original languageEnglish
Pages (from-to)2669-2677
Number of pages9
JournalProteomics
Volume15
Issue number15
DOIs
StatePublished - 1 Aug 2015

Keywords

  • Animal proteomics
  • Fetal programming
  • Obesity
  • Secrenin 1
  • Ubiquitin carboxy-terminal hydrolase L1

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