Protein kinase A regulates the osteogenic activity of Osterix

Siyuan He; You Hee Choi; Joong Kook Choi; Chang Yeol Yeo; Changju Chun; Kwang Youl Lee

doi:10.1002/jcb.24851

Protein kinase A regulates the osteogenic activity of Osterix

Siyuan He, You Hee Choi, Joong Kook Choi, Chang Yeol Yeo, Changju Chun, Kwang Youl Lee

Department of Life Science

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Osterix belongs to the SP gene family and is a core transcription factor responsible for osteoblast differentiation and bone formation. Activation of protein kinase A (PKA), a serine/threonine kinase, is essential for controlling bone formation and BMP-induced osteoblast differentiation. However, the relationship between Osterix and PKA is still unclear. In this report, we investigated the precise role of the PKA pathway in regulating Osterix during osteoblast differentiation. We found that PKA increased the protein level of Osterix; PKA phosphorylated Osterix, increased protein stability, and enhanced the transcriptional activity of Osterix. These results suggest that Osterix is a novel target of PKA, and PKA modulates osteoblast differentiation partially through the regulation of Osterix.

Original language	English
Pages (from-to)	1808-1815
Number of pages	8
Journal	Journal of Cellular Biochemistry
Volume	115
Issue number	10
DOIs	https://doi.org/10.1002/jcb.24851
State	Published - Oct 2014

Keywords

OSTEOBLAST DIFFERENTIATION
OSTERIX
PROTEIN KINASE A
PROTEIN STABILITY
TRANSCRIPTIONAL ACTIVITY

Access to Document

10.1002/jcb.24851

Cite this

@article{d6df43f39d134ddaa2edd92aef16abad,

title = "Protein kinase A regulates the osteogenic activity of Osterix",

abstract = "Osterix belongs to the SP gene family and is a core transcription factor responsible for osteoblast differentiation and bone formation. Activation of protein kinase A (PKA), a serine/threonine kinase, is essential for controlling bone formation and BMP-induced osteoblast differentiation. However, the relationship between Osterix and PKA is still unclear. In this report, we investigated the precise role of the PKA pathway in regulating Osterix during osteoblast differentiation. We found that PKA increased the protein level of Osterix; PKA phosphorylated Osterix, increased protein stability, and enhanced the transcriptional activity of Osterix. These results suggest that Osterix is a novel target of PKA, and PKA modulates osteoblast differentiation partially through the regulation of Osterix.",

keywords = "OSTEOBLAST DIFFERENTIATION, OSTERIX, PROTEIN KINASE A, PROTEIN STABILITY, TRANSCRIPTIONAL ACTIVITY",

author = "Siyuan He and Choi, {You Hee} and Choi, {Joong Kook} and Yeo, {Chang Yeol} and Changju Chun and Lee, {Kwang Youl}",

year = "2014",

month = oct,

doi = "10.1002/jcb.24851",

language = "English",

volume = "115",

pages = "1808--1815",

journal = "Journal of Cellular Biochemistry",

issn = "0730-2312",

publisher = "Wiley-Liss Inc.",

number = "10",

}

TY - JOUR

T1 - Protein kinase A regulates the osteogenic activity of Osterix

AU - He, Siyuan

AU - Choi, You Hee

AU - Choi, Joong Kook

AU - Yeo, Chang Yeol

AU - Chun, Changju

AU - Lee, Kwang Youl

PY - 2014/10

Y1 - 2014/10

N2 - Osterix belongs to the SP gene family and is a core transcription factor responsible for osteoblast differentiation and bone formation. Activation of protein kinase A (PKA), a serine/threonine kinase, is essential for controlling bone formation and BMP-induced osteoblast differentiation. However, the relationship between Osterix and PKA is still unclear. In this report, we investigated the precise role of the PKA pathway in regulating Osterix during osteoblast differentiation. We found that PKA increased the protein level of Osterix; PKA phosphorylated Osterix, increased protein stability, and enhanced the transcriptional activity of Osterix. These results suggest that Osterix is a novel target of PKA, and PKA modulates osteoblast differentiation partially through the regulation of Osterix.

AB - Osterix belongs to the SP gene family and is a core transcription factor responsible for osteoblast differentiation and bone formation. Activation of protein kinase A (PKA), a serine/threonine kinase, is essential for controlling bone formation and BMP-induced osteoblast differentiation. However, the relationship between Osterix and PKA is still unclear. In this report, we investigated the precise role of the PKA pathway in regulating Osterix during osteoblast differentiation. We found that PKA increased the protein level of Osterix; PKA phosphorylated Osterix, increased protein stability, and enhanced the transcriptional activity of Osterix. These results suggest that Osterix is a novel target of PKA, and PKA modulates osteoblast differentiation partially through the regulation of Osterix.

KW - OSTEOBLAST DIFFERENTIATION

KW - OSTERIX

KW - PROTEIN KINASE A

KW - PROTEIN STABILITY

KW - TRANSCRIPTIONAL ACTIVITY

UR - http://www.scopus.com/inward/record.url?scp=84906095594&partnerID=8YFLogxK

U2 - 10.1002/jcb.24851

DO - 10.1002/jcb.24851

M3 - Article

C2 - 24905700

AN - SCOPUS:84906095594

SN - 0730-2312

VL - 115

SP - 1808

EP - 1815

JO - Journal of Cellular Biochemistry

JF - Journal of Cellular Biochemistry

IS - 10

ER -

Protein kinase A regulates the osteogenic activity of Osterix

Abstract

Keywords

Access to Document

Fingerprint

Cite this