Protective effects of silkworm hemolymph extract and its fractions on UV-induced photoaging

Ju Hyun Park, Ji Eun Lee, Shin Sik Choi, Tai Hyun Park

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12 Scopus citations


Ultraviolet (UV) irradiation induces skin photoaging by generating reactive oxygen species (ROS). ROS caused by UV-irradiation results in loss of skin cells and degradation of extracellular matrix. A number of antioxidants have been chemically synthesized or naturally extracted to prevent ROS-mediated skin photoaging. In our previous work, silkworm hemolymph extract (SHEX) was prepared, and its antioxidant activity was tested by free radical-scavenging assay. This study assessed the protective effects of SHEX on UV-induced photoaging of human immortalized keratinocytes (HaCaT). UVA (365 nm)-induced ROS generation was inhibited by supplementation of silkworm hemolymph (SH). Treatment with SHEX prepared by boiling SH inhibited death of HaCaT cells caused by UVB (315 nm) and UVA irradiation in a dose-dependent manner. Seven fractions were obtained by separating SHEX by gel permeation chromatography and the antioxidant activity of the fractions was examined. The fraction showing the highest protective efficacy on UV-induced cell damage corresponded to the lutein-containing fraction isolated in our previous study. Moreover, the SHEX fraction suppressed the expression of MMP-1 (matrix metalloproteinase-1), a matrix-degrading enzyme, suggesting that the active constituent of SHEX has the potential to inhibit skin photoaging. These results suggest that SHEX can be developed as a dietary and cosmetic supplement for prevention of skin photoaging.

Original languageEnglish
Pages (from-to)37-44
Number of pages8
JournalBiotechnology and Bioprocess Engineering
Issue number1
StatePublished - 1 Feb 2017

Bibliographical note

Publisher Copyright:
© 2017, The Korean Society for Biotechnology and Bioengineering and Springer-Verlag Berlin Heidelberg.


  • antioxidant
  • reactive oxygen species (ROS)
  • Silkworm hemolymph extract (SHEX)
  • skin photoaging
  • UV irradiation


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