The protective properties of irisolidone (a metabolite of kakkalide by intestinal bacteria) against hydrogen peroxide (H2O2) induced cell damage were investigated. Irisolidone was found to scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, and the intracellular reactive oxygen species (ROS), thereby preventing lipid peroxidation and DNA damage. Irisolidone inhibited apoptosis in Chinese hamster lung fibroblast (V79-4) cells induced by H2O2 via radical scavenging activity. This was achieved by the activation of the extracellular signal regulated kinase (ERK) and DNA binding activity of activator protein-1 (AP-1) (a downstream transcription factor of ERK) by irisolidone.
Bibliographical noteFunding Information:
This research was supported by the study of the DNA repair regulation with the disease program and by the program of Basic Atomic Energy Research Institute (BAERI) which is a part of the Nuclear R&D programs grant from the Ministry of Science and Technology of Korea.
- Extracellular signal regulated kinase
- Oxidative stress
- Reactive oxygen species