Production of polyclonal antibodies against the tegument of sparganum (Plerocercoid of Spirometra mansoni) and its immunolocalization

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Abstract

In a previous study, the author developed a method for separation of the tegument of spargana (plerocercoids of Spirometra mansoni) from the parenchyme using urea. The present study, as a next step, was performed to evaluate which molecules are present in the outer tegument. Two major proteins, 180 and 200 kDa, are present in the tegument and we could make polyclonal antibodies against these molecules. Their immunolocalization was processed and the outermost layer of the spargana showed strong positive staining. Conclusively, we could confirm that the 180 and 200 kDa molecules might be tightly bound membrane proteins in the tegument of spargana.

Original languageEnglish
Pages (from-to)183-185
Number of pages3
JournalThe Korean journal of parasitology
Volume48
Issue number2
DOIs
StatePublished - Jun 2010

Keywords

  • Immunohistochemistry
  • Polyclonal antibody
  • Sparganum
  • Syncytial layer

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