Production of poly(3-hydroxybutyrate) from whey by cell recycle fed-batch culture of recombinant Escherichia coli

Woo Suk Ahn, Si Jae Park, Sang Yup Lee

Research output: Contribution to journalArticlepeer-review

102 Scopus citations

Abstract

A new fermentation strategy using cell recycle membrane system was developed for the efficient production of poly(3-hydroxybutyrate) (PHB) from whey by recombinant Escherichia coli strain CGSC 4401 harboring the Alcaligenes latus polyhydroxyalkanoate (PHA) biosynthesis genes. By cell recycle, red-batch cultivation employing an external membrane module, the working volume of fermentation could be constantly maintained at 2.3 1. The final cell concentration, PHB concentration and PHB content of 194 g 1-1, 168 g 1-1 and 87%, respectively, were obtained in 36.5 h by the pH-stat cell recycle fed-batch culture using whey solution concentrated to contain 280 g lactose 1-1 as a feeding solution, resulting in a high productivity of 4.6 g PHB 1-1 h-1.

Original languageEnglish
Pages (from-to)235-240
Number of pages6
JournalBiotechnology Letters
Volume23
Issue number3
DOIs
StatePublished - 2001

Keywords

  • Cell recycle fed-batch culture
  • Poly(3-hydroxybutyrate)
  • Recombinant E. coli
  • Whey

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