Production of poly(3-hydroxybutyrate) from whey by cell recycle fed-batch culture of recombinant Escherichia coli

Woo Suk Ahn; Si Jae Park; Sang Yup Lee

doi:10.1023/A:1005633418161

Production of poly(3-hydroxybutyrate) from whey by cell recycle fed-batch culture of recombinant Escherichia coli

Woo Suk Ahn, Si Jae Park, Sang Yup Lee

Chemical Engineering & Materials Science

Research output: Contribution to journal › Article › peer-review

85 Scopus citations

Abstract

A new fermentation strategy using cell recycle membrane system was developed for the efficient production of poly(3-hydroxybutyrate) (PHB) from whey by recombinant Escherichia coli strain CGSC 4401 harboring the Alcaligenes latus polyhydroxyalkanoate (PHA) biosynthesis genes. By cell recycle, red-batch cultivation employing an external membrane module, the working volume of fermentation could be constantly maintained at 2.3 1. The final cell concentration, PHB concentration and PHB content of 194 g 1^-1, 168 g 1^-1 and 87%, respectively, were obtained in 36.5 h by the pH-stat cell recycle fed-batch culture using whey solution concentrated to contain 280 g lactose 1^-1 as a feeding solution, resulting in a high productivity of 4.6 g PHB 1^-1 h^-1.

Original language	English
Pages (from-to)	235-240
Number of pages	6
Journal	Biotechnology Letters
Volume	23
Issue number	3
DOIs	https://doi.org/10.1023/A:1005633418161
State	Published - 2001

Keywords

Cell recycle fed-batch culture
Poly(3-hydroxybutyrate)
Recombinant E. coli
Whey

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10.1023/A:1005633418161

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title = "Production of poly(3-hydroxybutyrate) from whey by cell recycle fed-batch culture of recombinant Escherichia coli",

abstract = "A new fermentation strategy using cell recycle membrane system was developed for the efficient production of poly(3-hydroxybutyrate) (PHB) from whey by recombinant Escherichia coli strain CGSC 4401 harboring the Alcaligenes latus polyhydroxyalkanoate (PHA) biosynthesis genes. By cell recycle, red-batch cultivation employing an external membrane module, the working volume of fermentation could be constantly maintained at 2.3 1. The final cell concentration, PHB concentration and PHB content of 194 g 1-1, 168 g 1-1 and 87%, respectively, were obtained in 36.5 h by the pH-stat cell recycle fed-batch culture using whey solution concentrated to contain 280 g lactose 1-1 as a feeding solution, resulting in a high productivity of 4.6 g PHB 1-1 h-1.",

keywords = "Cell recycle fed-batch culture, Poly(3-hydroxybutyrate), Recombinant E. coli, Whey",

author = "Ahn, {Woo Suk} and Park, {Si Jae} and Lee, {Sang Yup}",

note = "Funding Information: We thank Mary Berlyn for kindly providing the CGSC strains. This work was supported by the Korea-Australia International Cooperative Project from the Ministry of Science and Technology (MOST).",

year = "2001",

doi = "10.1023/A:1005633418161",

language = "English",

volume = "23",

pages = "235--240",

journal = "Biotechnology Letters",

issn = "0141-5492",

publisher = "Springer Netherlands",

number = "3",

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TY - JOUR

T1 - Production of poly(3-hydroxybutyrate) from whey by cell recycle fed-batch culture of recombinant Escherichia coli

AU - Ahn, Woo Suk

AU - Park, Si Jae

AU - Lee, Sang Yup

N1 - Funding Information: We thank Mary Berlyn for kindly providing the CGSC strains. This work was supported by the Korea-Australia International Cooperative Project from the Ministry of Science and Technology (MOST).

PY - 2001

Y1 - 2001

N2 - A new fermentation strategy using cell recycle membrane system was developed for the efficient production of poly(3-hydroxybutyrate) (PHB) from whey by recombinant Escherichia coli strain CGSC 4401 harboring the Alcaligenes latus polyhydroxyalkanoate (PHA) biosynthesis genes. By cell recycle, red-batch cultivation employing an external membrane module, the working volume of fermentation could be constantly maintained at 2.3 1. The final cell concentration, PHB concentration and PHB content of 194 g 1-1, 168 g 1-1 and 87%, respectively, were obtained in 36.5 h by the pH-stat cell recycle fed-batch culture using whey solution concentrated to contain 280 g lactose 1-1 as a feeding solution, resulting in a high productivity of 4.6 g PHB 1-1 h-1.

AB - A new fermentation strategy using cell recycle membrane system was developed for the efficient production of poly(3-hydroxybutyrate) (PHB) from whey by recombinant Escherichia coli strain CGSC 4401 harboring the Alcaligenes latus polyhydroxyalkanoate (PHA) biosynthesis genes. By cell recycle, red-batch cultivation employing an external membrane module, the working volume of fermentation could be constantly maintained at 2.3 1. The final cell concentration, PHB concentration and PHB content of 194 g 1-1, 168 g 1-1 and 87%, respectively, were obtained in 36.5 h by the pH-stat cell recycle fed-batch culture using whey solution concentrated to contain 280 g lactose 1-1 as a feeding solution, resulting in a high productivity of 4.6 g PHB 1-1 h-1.

KW - Cell recycle fed-batch culture

KW - Poly(3-hydroxybutyrate)

KW - Recombinant E. coli

KW - Whey

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U2 - 10.1023/A:1005633418161

DO - 10.1023/A:1005633418161

M3 - Article

AN - SCOPUS:0035133622

SN - 0141-5492

VL - 23

SP - 235

EP - 240

JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 3

ER -

Production of poly(3-hydroxybutyrate) from whey by cell recycle fed-batch culture of recombinant Escherichia coli

Abstract

Keywords

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