Abstract
A new fermentation strategy using cell recycle membrane system was developed for the efficient production of poly(3-hydroxybutyrate) (PHB) from whey by recombinant Escherichia coli strain CGSC 4401 harboring the Alcaligenes latus polyhydroxyalkanoate (PHA) biosynthesis genes. By cell recycle, red-batch cultivation employing an external membrane module, the working volume of fermentation could be constantly maintained at 2.3 1. The final cell concentration, PHB concentration and PHB content of 194 g 1-1, 168 g 1-1 and 87%, respectively, were obtained in 36.5 h by the pH-stat cell recycle fed-batch culture using whey solution concentrated to contain 280 g lactose 1-1 as a feeding solution, resulting in a high productivity of 4.6 g PHB 1-1 h-1.
| Original language | English |
|---|---|
| Pages (from-to) | 235-240 |
| Number of pages | 6 |
| Journal | Biotechnology Letters |
| Volume | 23 |
| Issue number | 3 |
| DOIs | |
| State | Published - 2001 |
Bibliographical note
Funding Information:We thank Mary Berlyn for kindly providing the CGSC strains. This work was supported by the Korea-Australia International Cooperative Project from the Ministry of Science and Technology (MOST).
Keywords
- Cell recycle fed-batch culture
- Poly(3-hydroxybutyrate)
- Recombinant E. coli
- Whey