Production of human spinal-cord organoids recapitulating neural-tube morphogenesis

Ju Hyun Lee; Hyogeun Shin; Mohammed R. Shaker; Hyun Jung Kim; Si Hyung Park; June Hoan Kim; Namwon Lee; Minjin Kang; Subin Cho; Tae Hwan Kwak; Jong Woon Kim; Mi Ryoung Song; Seung Hae Kwon; Dong Wook Han; Sanghyuk Lee; Se Young Choi; Im Joo Rhyu; Hyun Kim; Dongho Geum; Il Joo Cho; Woong Sun

doi:10.1038/s41551-022-00868-4

Production of human spinal-cord organoids recapitulating neural-tube morphogenesis

Ju Hyun Lee, Hyogeun Shin, Mohammed R. Shaker, Hyun Jung Kim, Si Hyung Park, June Hoan Kim, Namwon Lee, Minjin Kang, Subin Cho, Tae Hwan Kwak, Jong Woon Kim, Mi Ryoung Song, Seung Hae Kwon, Dong Wook Han, Sanghyuk Lee, Se Young Choi, Im Joo Rhyu, Hyun Kim, Dongho Geum, Il Joo ChoWoong Sun

Department of Life Science

Research output: Contribution to journal › Article › peer-review

58 Scopus citations

Abstract

Human spinal-cord-like tissues induced from human pluripotent stem cells are typically insufficiently mature and do not mimic the morphological features of neurulation. Here, we report a three-dimensional culture system and protocol for the production of human spinal-cord-like organoids (hSCOs) recapitulating the neurulation-like tube-forming morphogenesis of the early spinal cord. The hSCOs exhibited neurulation-like tube-forming morphogenesis, cellular differentiation into the major types of spinal-cord neurons as well as glial cells, and mature synaptic functional activities, among other features of the development of the spinal cord. We used the hSCOs to screen for antiepileptic drugs that can cause neural-tube defects. hSCOs may also facilitate the study of the development of the human spinal cord and the modelling of diseases associated with neural-tube defects.

Original language	English
Pages (from-to)	435-448
Number of pages	14
Journal	Nature Biomedical Engineering
Volume	6
Issue number	4
DOIs	https://doi.org/10.1038/s41551-022-00868-4
State	Published - Apr 2022

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Publisher Copyright:
© 2022, The Author(s), under exclusive licence to Springer Nature Limited.

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10.1038/s41551-022-00868-4

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Lee, J. H., Shin, H., Shaker, M. R., Kim, H. J., Park, S. H., Kim, J. H., Lee, N., Kang, M., Cho, S., Kwak, T. H., Kim, J. W., Song, M. R., Kwon, S. H., Han, D. W., Lee, S., Choi, S. Y., Rhyu, I. J., Kim, H., Geum, D., ... Sun, W. (2022). Production of human spinal-cord organoids recapitulating neural-tube morphogenesis. Nature Biomedical Engineering, 6(4), 435-448. https://doi.org/10.1038/s41551-022-00868-4

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title = "Production of human spinal-cord organoids recapitulating neural-tube morphogenesis",

abstract = "Human spinal-cord-like tissues induced from human pluripotent stem cells are typically insufficiently mature and do not mimic the morphological features of neurulation. Here, we report a three-dimensional culture system and protocol for the production of human spinal-cord-like organoids (hSCOs) recapitulating the neurulation-like tube-forming morphogenesis of the early spinal cord. The hSCOs exhibited neurulation-like tube-forming morphogenesis, cellular differentiation into the major types of spinal-cord neurons as well as glial cells, and mature synaptic functional activities, among other features of the development of the spinal cord. We used the hSCOs to screen for antiepileptic drugs that can cause neural-tube defects. hSCOs may also facilitate the study of the development of the human spinal cord and the modelling of diseases associated with neural-tube defects.",

author = "Lee, {Ju Hyun} and Hyogeun Shin and Shaker, {Mohammed R.} and Kim, {Hyun Jung} and Park, {Si Hyung} and Kim, {June Hoan} and Namwon Lee and Minjin Kang and Subin Cho and Kwak, {Tae Hwan} and Kim, {Jong Woon} and Song, {Mi Ryoung} and Kwon, {Seung Hae} and Han, {Dong Wook} and Sanghyuk Lee and Choi, {Se Young} and Rhyu, {Im Joo} and Hyun Kim and Dongho Geum and Cho, {Il Joo} and Woong Sun",

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doi = "10.1038/s41551-022-00868-4",

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Lee, JH, Shin, H, Shaker, MR, Kim, HJ, Park, SH, Kim, JH, Lee, N, Kang, M, Cho, S, Kwak, TH, Kim, JW, Song, MR, Kwon, SH, Han, DW, Lee, S, Choi, SY, Rhyu, IJ, Kim, H, Geum, D, Cho, IJ & Sun, W 2022, 'Production of human spinal-cord organoids recapitulating neural-tube morphogenesis', Nature Biomedical Engineering, vol. 6, no. 4, pp. 435-448. https://doi.org/10.1038/s41551-022-00868-4

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AU - Kim, June Hoan

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AU - Kang, Minjin

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AU - Kwon, Seung Hae

AU - Han, Dong Wook

AU - Lee, Sanghyuk

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AU - Rhyu, Im Joo

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AU - Sun, Woong

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Production of human spinal-cord organoids recapitulating neural-tube morphogenesis

Abstract

Bibliographical note

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