Production of human spinal-cord organoids recapitulating neural-tube morphogenesis

Ju Hyun Lee, Hyogeun Shin, Mohammed R. Shaker, Hyun Jung Kim, Si Hyung Park, June Hoan Kim, Namwon Lee, Minjin Kang, Subin Cho, Tae Hwan Kwak, Jong Woon Kim, Mi Ryoung Song, Seung Hae Kwon, Dong Wook Han, Sanghyuk Lee, Se Young Choi, Im Joo Rhyu, Hyun Kim, Dongho Geum, Il Joo ChoWoong Sun

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Human spinal-cord-like tissues induced from human pluripotent stem cells are typically insufficiently mature and do not mimic the morphological features of neurulation. Here, we report a three-dimensional culture system and protocol for the production of human spinal-cord-like organoids (hSCOs) recapitulating the neurulation-like tube-forming morphogenesis of the early spinal cord. The hSCOs exhibited neurulation-like tube-forming morphogenesis, cellular differentiation into the major types of spinal-cord neurons as well as glial cells, and mature synaptic functional activities, among other features of the development of the spinal cord. We used the hSCOs to screen for antiepileptic drugs that can cause neural-tube defects. hSCOs may also facilitate the study of the development of the human spinal cord and the modelling of diseases associated with neural-tube defects.

Original languageEnglish
Pages (from-to)435-448
Number of pages14
JournalNature Biomedical Engineering
Volume6
Issue number4
DOIs
StatePublished - Apr 2022

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